Document Detail

Quantitation of epidermal and mucosal tissue injury using contrast agents and imaging techniques.
MedLine Citation:
PMID:  19622128     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/PURPOSE: Epidermal injury is common but the accuracy of visual methods is significantly impacted by the inherent skin pigmentation. We examined imaging and fluorescence techniques to quantify tissue injury as a function of skin color.
METHODS: Epidermal and mucosal scratches were created in 20 light- (L(*) 68.2+/-2.3) and 20 dark-skinned (L(*) 46.4+/-5.2) females. Injured and uninjured sites were treated with toluidine blue (TB), fluorescein (FL) and a TB/FL mixture and photographed under conditions of white and fluorescent light. Area and intensity parameters were determined.
RESULTS: Injured sites with TB and TB/FL had higher areas than the control for both light and dark subjects (ANOVA, P<0.05). The intensity of the injured TB site was higher than the control for light skin only. The areas of injured sites with FL and TB/FL were higher than the control for both groups as were the intensities of the injured sites with FL. The findings were similar for the lip skin.
CONCLUSIONS: Application of TB and FL contrasts under white and fluorescent light can be used to quantify tissue injuries for L(*) values >35 and is a promising approach for the quantitation across a range of skin pigmentation.
Marty O Visscher; David Sullivan; Steven Sullivan; Brian Barford; Murray Dock; Marilyn S Sommers
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)     Volume:  15     ISSN:  1600-0846     ISO Abbreviation:  Skin Res Technol     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-07-22     Completed Date:  2009-10-08     Revised Date:  2011-07-27    
Medline Journal Info:
Nlm Unique ID:  9504453     Medline TA:  Skin Res Technol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  180-6     Citation Subset:  IM    
Cincinnati Children's Hospital Medical Center, The Skin Sciences Institute, Cincinnati, OH 45229, USA.
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MeSH Terms
Contrast Media
Dermoscopy / methods*
Epidermis / injuries*,  pathology*
Image Enhancement / methods*
Microscopy, Fluorescence / methods*
Middle Aged
Mucous Membrane / injuries*,  pathology*
Pigments, Biological / analysis*
Grant Support
Reg. No./Substance:
0/Contrast Media; 0/Pigments, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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