| Quantitation of combinatorial libraries of small organic molecules by normal-phase HPLC with evaporative light-scattering detection. | |
| | |
MedLine Citation:
|
PMID: 9237216 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The advantages of evaporative light-scattering detection over UV detection for the quantitation of combinatorial libraries composed of small organic compounds by HPLC are described. The detector's response is independent of the sample chromophore, which makes it well-suited to chromatographic analyses of mixtures of dissimilar solutes. Thus, HPLC with evaporative light-scattering detection offers to potential for reducing false positive or false negative results in screening assays, because of its ability to detect the presence of impurities that absorb poorly in the UV (e.g., those impurities originating from the polymeric support). Furthermore, the evaporative light-scattering detector exhibits a nearly equivalent response to compounds of similar structural class. Hence, rapid quantitation of compound libraries may be carried out with the use of a single external standard. For example, the quantitation errors, based on a single external standard, for a series of steroids, hydantoins, and BOC- and Fmoc-protected amino acids by normal-phase HPLC with evaporative light-scattering detection average approximately +/-10%. The application of the evaporative light-scattering detector to the quantitation of low-level sample impurities and the detector's compatibility with gradient elution are also described. |
| | |
Authors:
|
C E Kibbey |
Related Documents
:
|
6853656 - Applications of rapid-scanning multichannel detectors in chromatography. plenary lecture. 19913236 - A free-flowing soap film combined with cavity ring-down spectroscopy as a detection sys... 10644856 - Recent advances in liquid-phase combinatorial chemistry. 6846816 - Chromatographic analysis of sugars in physiological fluids by postcolumn reaction with ... 23217306 - Separation of intermediates of iron-catalyzed dopamine oxidation reactions using revers... 16705646 - Utility of porous graphitic carbon stationary phase in quantitative liquid chromatograp... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Molecular diversity Volume: 1 ISSN: 1381-1991 ISO Abbreviation: Mol. Divers. Publication Date: 1996 Aug |
Date Detail:
|
Created Date: 1997-09-23 Completed Date: 1997-09-23 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9516534 Medline TA: Mol Divers Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 247-58 Citation Subset: IM |
Affiliation:
|
Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, MI 48105, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
chemistry Chemistry, Organic / methods Chromatography, High Pressure Liquid / methods*, standards Directed Molecular Evolution / methods* Hydantoins / chemistry Light Reference Standards Scattering, Radiation Spectrophotometry, Ultraviolet Steroids / chemistry, standards |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Hydantoins; 0/Steroids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Analysis of novel streptavidin-binding peptides, identified using a phage display library, shows tha...
Next Document: Identification of cyclized calmodulin antagonists from a phage display random peptide library.