| Quantitation of ceramides in nude mouse skin by normal-phase liquid chromatography and atmospheric pressure chemical ionization mass spectrometry. | |
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MedLine Citation:
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PMID: 20153711 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive and accurate normal-phase liquid chromatography and atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS) method for determining the standard ceramide [NS] (Cer[NS]) was developed and validated so as to improve the traditional thin-layer chromatography (TLC) technique and LC-electrospray ionization (ESI)-MS method to profile and quantify ceramides in nude mouse skin. Normal-phase LC-APCI-MS was optimized to separate the nine classes of ceramides presented in the stratum corneum (SC) of nude mouse skin. A normal-phase silica column eluted with the gradient system from heptane:acetone/butanol (90:10, v/v) of 75:25 to 100% acetone/butanol (90:10, v/v) (with each solvent containing 0.1% [v/v] triethylamine and 0.1% [v/v] formic acid) at a flow rate of 0.8 ml/min was found to be optimal for analyzing standard Cer[NS]. The analysis of Cer[NS] was validated and employed as the standard for constructing a calibration curve to quantitate all classes of ceramides. This method was applied to profile the classes and contents of ceramides in the SC of nude mouse skin and proved to be workable. It was concluded that this improved method can be used to directly detect and quantify all classes of ceramides in the SC of nude mouse skin and that it is more convenient and labor-saving than the traditional TLC method. |
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Authors:
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Yi-Bo Liou; Ming-Thau Sheu; Der-Zen Liu; Shan-Yang Lin; Hsiu-O Ho |
Publication Detail:
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Type: Journal Article Date: 2010-02-12 |
Journal Detail:
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Title: Analytical biochemistry Volume: 401 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-12 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 107-13 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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College of Pharmacy, Taipei Medical University, Taipei 110, Taiwan, ROC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atmospheric Pressure Ceramides / analysis*, chemistry Chromatography, High Pressure Liquid / methods* Mice Mice, Nude Skin / metabolism* Spectrometry, Mass, Electrospray Ionization / methods* |
| Chemical | |
Reg. No./Substance:
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0/Ceramides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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