| Quantitation of branched-chain amino acids in ascites by capillary electrophoresis with light-emitting diode-induced fluorescence detection. | |
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MedLine Citation:
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PMID: 21449064 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Branched-chain amino acids (BCAAs) are one of the important biomarkers for monitoring liver disease such as hepatitis or hepatoma. In this communication, we present the determination of the concentrations of BCAA in ascites by CE light-emitted diode-induced fluorescence (LEDIF) using 1.5% m/v poly(ethylene oxide) (average M(v) : ∼8 000 000 g/mol) that was prepared in 10 mM sodium tetraborate solution (pH 9.3). Naphthalene-2,3-dicarboxaldehyde was used to derivatize 15 amino acids (AAs) to form naphthalene-2,3-dicarboxaldehyde (NDA)-AA derivatives prior to CE analysis. The separation of 15 NDA-AA derivatives was accomplished within 15 min, with RSD values of <5.8% (within-day) and 7.4% (between-days) with respect to their migration times. The limits of detection for the tested BCAAs ranged from 10.6 to 10.9 nM. We determined the concentrations of three BCAAs - leucine, isoleucine and valine - in ascites by applying a standard addition method, with recovery percentages ranging from 93.9 to 111%. The results obtained from this CE-LEDIF method is in good agreement with those by a gold standard method using an AA analyzer. We have found that the concentrations of the three BCAAs in ascites obtained from patients suffering from liver diseases were lower than those from healthy individuals. Our approach is highly efficient, sensitive, and cost-effective, which holds great potential for the diagnosis of liver diseases. |
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Authors:
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Po-Ling Chang; Tai-Chia Chiu; Tsang-En Wang; Kuang-Chun Hu; Yu-Hsien Tsai; Cho-Chun Hu; Ming-Jong Bair; Huan-Tsung Chang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-30 |
Journal Detail:
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Title: Electrophoresis Volume: - ISSN: 1522-2683 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Department of Chemistry, Tunghai University, Taichung, Taiwan. poling@thu.edu.tw. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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