Document Detail


Quantitation of alpha-factor internalization and response during the Saccharomyces cerevisiae cell cycle.
MedLine Citation:
PMID:  1656226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The alpha-factor pheromone binds to specific cell surface receptors on Saccharomyces cerevisiae a cells. The pheromone is then internalized, and cell surface receptors are down-regulated. At the same time, a signal is transmitted that causes changes in gene expression and cell cycle arrest. We show that the ability of cells to internalize alpha-factor is constant throughout the cell cycle, a cells are also able to respond to pheromone throughout the cycle even though there is cell cycle modulation of the expression of two pheromone-inducible genes, FUS1 and STE2. Both of these genes are expressed less efficiently near or just after the START point of the cell cycle in response to alpha-factor. For STE2, the basal level of expression is modulated in the same manner.
Authors:
B Zanolari; H Riezman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  11     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1991 Oct 
Date Detail:
Created Date:  1991-10-31     Completed Date:  1991-10-31     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5251-8     Citation Subset:  IM    
Affiliation:
Biocenter, University of Basel, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle
Endocytosis
Gene Expression Regulation, Fungal*
Genes, Fungal / genetics
Kinetics
Microscopy, Fluorescence
Peptides / metabolism*,  pharmacology
Receptors, Cell Surface / genetics,  metabolism
Receptors, Mating Factor
Receptors, Peptide*
Saccharomyces cerevisiae / cytology,  genetics,  metabolism*
Signal Transduction
Transcription Factors*
Chemical
Reg. No./Substance:
0/Peptides; 0/Receptors, Cell Surface; 0/Receptors, Mating Factor; 0/Receptors, Peptide; 0/Transcription Factors; 61194-02-3/mating factor
Comments/Corrections

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