Document Detail


Quantitation of Giardia cysts and Cryptosporidium oocysts in fecal samples by direct immunofluorescence assay.
MedLine Citation:
PMID:  8263179     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lack of quick, simple, and sensitive quantitative tests has impeded studies on infection patterns and treatment of Giardia spp. and Cryptosporidium spp. A quantitative direct immunofluorescence assay (FA) using a commercial FA kit was developed and evaluated. Recovery rates of the FA for Cryptosporidium oocysts in calf feces seeded with 1,000, 10,000, 100,000, and 1,000,000 oocysts per g were 14.8, 40.8, 84.2, and 78.2%, respectively. Interassay coefficients of variation were 10.6 to 47.1%. Recovery rates of the FA for Giardia cysts in feces seeded with 1,000, 10,000, and 100,000 cysts per g were 76.4, 96.9, and 89.6%, respectively. Interassay coefficients of variation were 7.4 to 22.1%. By comparison, recovery rates of Giardia cyst by sucrose gradient flotation were only 20.5, 51.2, and 42.9%, respectively. Counts of cysts-per-gram obtained by sucrose gradient flotation with samples from calves, lambs, and ewes were only 49.1 to 54.8% of those obtained by the FA. Zinc sulfate flotation detected only 36.4% of infections when there were < or = 1,000 cysts per g. The quantitative FA offers a useful technique for epidemiological and control studies of these two parasites.
Authors:
L Xiao; R P Herd
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  31     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-01-27     Completed Date:  1994-01-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2944-6     Citation Subset:  IM    
Affiliation:
Department of Veterinary Preventive Medicine, Ohio State University College of Veterinary Medicine, Columbus 43210.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cryptosporidium / isolation & purification*
Feces / parasitology*
Fluorescent Antibody Technique
Giardia / isolation & purification*
Parasite Egg Count / methods*
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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