| Quantitating staphylococcal enterotoxin B in diverse media using a portable fiber-optic biosensor. | |
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MedLine Citation:
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PMID: 8789146 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new, portable fiber-optic biosensor has been used to detect staphylococcal enterotoxin B, a causative agent of food poisoning, at levels as low as 0.5 ng/ml in buffer. The toxin (SEB) can also be detected and quantitated in other relevant media: human serum, urine, and aqueous extract of ham. The level of toxin, from 5 to 200 ng/ml, can be accurately predicted in these media by calibrating each fiber and by comparing results to a single standard curve based on toxin in buffer. The quantitative fluorescent sandwich immunoassay provides results in 45 min; qualitative results are provided in 15-20 min. Using a blender and a benchtop centrifuge, fast, simple aqueous extracts of contaminated ham samples were prepared and tested. Ham spiked with 5 or 40 micrograms SEB per 100 g food resulted in biosensor readings indicative of 11 or 69% recovery of the toxin, respectively. Finally, the SEB assay is highly specific; SEA and SED give only 2-3% of the signal at 5000 ng/ml as SEB gives at 1000 ng/ml. This specific, sensitive assay for SEB on the portable fiber-optic biosensor permits easy monitoring of clinical samples or on-site analysis of suspect food samples. |
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Authors:
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L A Tempelman; K D King; G P Anderson; F S Ligler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 233 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1996 Jan |
Date Detail:
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Created Date: 1996-10-21 Completed Date: 1996-10-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 50-7 Citation Subset: IM |
Affiliation:
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Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biosensing Techniques* Buffers Enterotoxins / analysis*, blood, urine Evaluation Studies as Topic Fiber Optic Technology* Fluoroimmunoassay / methods* Food Microbiology Humans Meat / analysis, microbiology Optical Fibers Staphylococcal Food Poisoning / blood, diagnosis, urine Staphylococcus aureus Swine |
| Chemical | |
Reg. No./Substance:
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0/Buffers; 0/Enterotoxins; 39424-53-8/enterotoxin B, staphylococcal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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