Document Detail


Quantitating glomerular endothelial fenestration: an unbiased stereological approach.
MedLine Citation:
PMID:  21659733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: Glomerular endothelial cells are fenestrated, allowing for especially high transcellular hydraulic conductivity. Current knowledge about endothelial fenestration structural changes in disease conditions is limited, partly due to the absence of robust methodologies to quantitate these structures. Herein, we propose a novel method for estimating the percentage of endothelial fenestration.
METHODS: An unbiased stereological method based on contiguity of two phases and surface area density estimation using isotropic uniform random line probes was developed. A line grid for intercept counting and classifying endothelial coverage of fenestrated versus non-fenestrated areas was designed. The method was applied to renal biopsies from 15 patients with Fabry disease and 9 normal living kidney donor controls.
RESULTS: The percentage of glomerular capillary endothelial coverage which was fenestrated was lower in Fabry patients (43 ± 12%) versus controls (53 ± 9%; p = 0.047). The fraction of endothelial surface which was fenestrated was greater on the peripheral versus mesangial zones of the capillary walls in both Fabry patients (p = 0.00002) and controls (p = 0.0005).
CONCLUSION: The proposed method provides an unbiased tool to quantitate endothelial fenestration changes in glomeruli. The practical example introduced showed reduced glomerular endothelial fenestration in Fabry nephropathy.
Authors:
Behzad Najafian; Michael Mauer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-10
Journal Detail:
Title:  American journal of nephrology     Volume:  33 Suppl 1     ISSN:  1421-9670     ISO Abbreviation:  Am. J. Nephrol.     Publication Date:  2011  
Date Detail:
Created Date:  2011-06-10     Completed Date:  2011-10-19     Revised Date:  2013-09-25    
Medline Journal Info:
Nlm Unique ID:  8109361     Medline TA:  Am J Nephrol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  34-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 S. Karger AG, Basel.
Affiliation:
Department of Pathology, University of Washington, Seattle, WA, USA. najafian@uw.edu
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Capillaries / pathology*,  ultrastructure
Child
Child, Preschool
Endothelium, Vascular / pathology*,  ultrastructure
Fabry Disease / pathology*
Female
Humans
Kidney Glomerulus / blood supply,  pathology*,  ultrastructure
Male
Microscopy, Electron, Transmission
Young Adult
Grant Support
ID/Acronym/Agency:
5U54NS065768/NS/NINDS NIH HHS; U54 NS065768/NS/NINDS NIH HHS
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