Document Detail

Quantifying the roles of cell motility and cell proliferation in a circular barrier assay.
MedLine Citation:
PMID:  23427098     Owner:  NLM     Status:  MEDLINE    
Moving fronts of cells are essential features of embryonic development, wound repair and cancer metastasis. This paper describes a set of experiments to investigate the roles of random motility and proliferation in driving the spread of an initially confined cell population. The experiments include an analysis of cell spreading when proliferation was inhibited. Our data have been analysed using two mathematical models: a lattice-based discrete model and a related continuum partial differential equation model. We obtain independent estimates of the random motility parameter, D, and the intrinsic proliferation rate, λ, and we confirm that these estimates lead to accurate modelling predictions of the position of the leading edge of the moving front as well as the evolution of the cell density profiles. Previous work suggests that systems with a high λ/D ratio will be characterized by steep fronts, whereas systems with a low λ/D ratio will lead to shallow diffuse fronts and this is confirmed in the present study. Our results provide evidence that continuum models, based on the Fisher-Kolmogorov equation, are a reliable platform upon which we can interpret and predict such experimental observations.
Matthew J Simpson; Katrina K Treloar; Benjamin J Binder; Parvathi Haridas; Kerry J Manton; David I Leavesley; D L Sean McElwain; Ruth E Baker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-02-20
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  10     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-02-21     Completed Date:  2013-08-12     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  20130007     Citation Subset:  IM    
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MeSH Terms
3T3 Cells
Cell Movement / physiology*
Cell Proliferation*
Models, Biological*

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