Document Detail


Quantifying recirculation in extracorporeal membrane oxygenation: a new technique validated.
MedLine Citation:
PMID:  20037890     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: The efficacy of veno-venous extracorporeal membrane oxygenation is limited by the phenomenon of recirculation, which is difficult to quantify. Existing measurement techniques using readily available equipment are unsatisfactory. OBJECTIVES: 1) To compare the accuracy of measurements of recirculation made using equations comparing blood oxygen content or saturation alone at different points in an ex vivo circuit; 2) to validate a new step-change technique for quantifying recirculation in vivo. METHODS: anesthetized greyhound dogs cannulated for veno-arterial support were connected to a circuit that allowed the creation of a known level of recirculation ex vivo and blood oxygen content/saturation monitoring. In two dogs, the accuracy of measurements derived from oxygen content and oxygen saturation were compared. The potential of a new technique for measuring recirculation in vivo by comparing the oxygen content of blood sampled during oxygenator bypass to that following a step-change in circuit oxygenation was demonstrated in a veno-venous pilot study and validated in a three-dog veno-arterial study. RESULTS: Measurements made using oxygen content versus oxygen saturation showed superior correlation with true recirculation (r(2)=0.87 vs. 0.64, p<0.0001) and less proportional measurement bias (10.3% vs. 49.8%, p=0.0045). Measurements of recirculation made using a step-change in circuit oxygenation and comparing oxygen content as is required for measuring in vivo recirculation overestimated by only 18.6% (95% Cl: 3.9-33.2%) and had excellent correlation with true values (r(2)=0.89). CONCLUSIONS: 1) Measurement of recirculation using oxygen content is superior to that using oxygen saturation alone, which demonstrates significant measurement bias; 2) the novel step-change technique is a sufficiently accurate technique for the measurement of recirculation in animal models.
Authors:
Steven J Lindstrom; Mark T Mennen; Franklin L Rosenfeldt; Robert F Salamonsen
Publication Detail:
Type:  Comparative Study; Journal Article; Validation Studies    
Journal Detail:
Title:  The International journal of artificial organs     Volume:  32     ISSN:  0391-3988     ISO Abbreviation:  Int J Artif Organs     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-28     Completed Date:  2010-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802649     Medline TA:  Int J Artif Organs     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  857-63     Citation Subset:  IM    
Affiliation:
Department of Epidemiology and Preventive Medicine, Monash University, Victoria, Australia. s.lindstrom@alfred.org.au
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / blood
Dogs
Extracorporeal Membrane Oxygenation / methods*
Femoral Artery*
Femoral Vein*
Models, Animal
Models, Biological*
Oxygen / blood*
Pilot Projects
Reproducibility of Results
Time Factors
Chemical
Reg. No./Substance:
0/Biological Markers; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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