Document Detail


Quantifying the influence of yellow fluorescent protein photoconversion on acceptor photobleaching-based fluorescence resonance energy transfer measurements.
MedLine Citation:
PMID:  22352644     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Fluorescence resonance energy transfer (FRET) efficiency measurements based on acceptor photobleaching of yellow fluorescent protein (YFP) are affected by the fact that bleaching of YFP produces a fluorescent species that is detectable in cyan fluorescent protein (CFP) image channels. The presented quantitative measurement of this conversion makes it possible to correct the obtained FRET signal to increase the accuracy of intensity based CFP/YFP FRET measurements. The described method can additionally be used to compare samples with very different fluorescence levels.
Authors:
Arne Seitz; Stefan Terjung; Timo Zimmermann; Rainer Pepperkok
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  17     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-02-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  011010     Citation Subset:  IM    
Affiliation:
European Molecular Biology Laboratory, Advanced Light Microscopy Facility, Meyerhofstr 1, 69117 Heidelberg, Germany.
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