| Quantifying cellular traction forces in three dimensions. | |
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MedLine Citation:
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PMID: 20018765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells engage in mechanical force exchange with their extracellular environment through tension generated by the cytoskeleton. A method combining laser scanning confocal microscopy (LSCM) and digital volume correlation (DVC) enables tracking and quantification of cell-mediated deformation of the extracellular matrix in all three spatial dimensions. Time-lapse confocal imaging of migrating 3T3 fibroblasts on fibronectin (FN)-modified polyacrylamide gels of varying thickness reveals significant in-plane (x, y) and normal (z) displacements, and illustrates the extent to which cells, even in nominally two-dimensional (2-D) environments, explore their surroundings in all three dimensions. The magnitudes of the measured displacements are independent of the elastic moduli of the gels. Analysis of the normal displacement profiles suggests that normal forces play important roles even in 2-D cell migration. |
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Authors:
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Stacey A Maskarinec; Christian Franck; David A Tirrell; Guruswami Ravichandran |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-12-15 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 106 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2010-01-18 Completed Date: 2010-02-17 Revised Date: 2010-09-27 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 22108-13 Citation Subset: IM |
Affiliation:
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Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, MC 210-41, Pasadena, CA 91125, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins Animals Bioengineering Biomechanics Cell Movement / physiology* Cytoskeleton / physiology Elasticity Extracellular Matrix / physiology Fibronectins / physiology Fluorescent Dyes Imaging, Three-Dimensional Mice Microscopy, Confocal Microspheres Models, Biological Swiss 3T3 Cells |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Fibronectins; 0/Fluorescent Dyes; 0/polyacrylamide gels |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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