| Quantifying the adhesion and interaction forces between Pseudomonas aeruginosa and natural organic matter. | |
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MedLine Citation:
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PMID: 18186333 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Atomic force microscopy (AFM) was used to characterize interactions between natural organic matter (NOM), and glass or bacteria. Poly(methacrylic acid) (PMA), soil humic Acid (SHA), and Suwannee River humic Acid (SRHA), were adsorbed to silica AFM probes. Adhesion forces (Fadh) for the interaction of organic-probes and glass slides correlated with organic molecular weight (MW), but not with radius of the organic aggregate (R), charge density (Q), or zeta potential (zeta). Two Pseudomonas aeruginosa strains with different lipopolysaccharides (LPS) were chosen: PAO1 (A+B+), whose LPS have common antigen (A-band) + O-antigen (B-band); and mutant AK1401 (A+B-). Fadh between bacteria and organics correlated with organic MW, R, and Q, but not zeta. PAO1 had lower Fadh with silica than NOM, which was attributed to negative charges from the B-band polymers causing electrostatic repulsion. AK1401 adhered stronger to silica than to the organics, perhaps because the absence of the B-band exposed underlying positively charged proteins. DLVO calculations could not explain the differences in the two bacteria or predict qualitative or quantitative trends in interaction forces in these systems. Molecular-level information from AFM studies can bring us closer to understanding the complex nature of bacterial-NOM interactions. |
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Authors:
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Laila I Abu-Lail; Yatao Liu; Arzu Atabek; Terri A Camesano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Environmental science & technology Volume: 41 ISSN: 0013-936X ISO Abbreviation: Environ. Sci. Technol. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2008-01-11 Completed Date: 2008-02-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0213155 Medline TA: Environ Sci Technol Country: United States |
Other Details:
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Languages: eng Pagination: 8031-7 Citation Subset: IM |
Affiliation:
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Department of Civil and Environmental Engineering, Life Sciences and Bioengineering Institute at Gateway Park, Worcester Polytechnic Institute, Worcester, MA 01609, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Adhesion* Humic Substances* Lipopolysaccharides / chemistry Microscopy, Atomic Force / methods Polymethacrylic Acids / chemistry Pseudomonas aeruginosa / chemistry*, physiology* Silicon Dioxide / chemistry Surface Properties Tissue Adhesions |
| Chemical | |
Reg. No./Substance:
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0/Humic Substances; 0/Lipopolysaccharides; 0/Polymethacrylic Acids; 7631-86-9/Silicon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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