Document Detail


Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation.
MedLine Citation:
PMID:  23150379     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Transcription factors control gene expression by binding to noncoding regions of DNA known as -cis-regulatory elements (CREs; i.e., enhancer/promoters). Traditionally, cis-regulatory analysis has been carried out via mouse transgenesis which is time-consuming and nonquantitative. Electroporation of DNA reporter constructs into living mouse tissue is a rapid and effective alternative to transgenesis which permits quantitative assessment of cis-regulatory activity. Here, we present a simple technique for quantifying the activity of photoreceptor-specific CREs in living explanted mouse retinas.
Authors:
Cynthia L Montana; Connie A Myers; Joseph C Corbo
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  935     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  329-340     Citation Subset:  -    
Affiliation:
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
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