| Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation. | |
| | |
MedLine Citation:
|
PMID: 23150379 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Transcription factors control gene expression by binding to noncoding regions of DNA known as -cis-regulatory elements (CREs; i.e., enhancer/promoters). Traditionally, cis-regulatory analysis has been carried out via mouse transgenesis which is time-consuming and nonquantitative. Electroporation of DNA reporter constructs into living mouse tissue is a rapid and effective alternative to transgenesis which permits quantitative assessment of cis-regulatory activity. Here, we present a simple technique for quantifying the activity of photoreceptor-specific CREs in living explanted mouse retinas. |
| | |
Authors:
|
Cynthia L Montana; Connie A Myers; Joseph C Corbo |
Related Documents
:
|
22174379 - Defining the structure of the general stress regulon of bacillus subtilis using targete... 16118439 - Study of transcriptional regulation using a reporter gene assay. 10196229 - A tata element is required for trna promoter activity and confers tata-binding protein ... 21987119 - A putative functional myb transcription factor induced by low temperature regulates ant... 18617999 - The generation of promoter-mediated transcriptional noise in bacteria. 17218319 - Pu.1 activates transcription of shp-1 gene in hematopoietic cells. 11173119 - Hla complex genes in type 1 diabetes and other autoimmune diseases. which genes are inv... 12448419 - Specimen block counter-staining for localization of gus expression in transgenic arabid... 10723559 - Use of fluorescent microspheres to localize in vivo gene transfer injection sites. |
Publication Detail:
|
Type: JOURNAL ARTICLE |
Journal Detail:
|
Title: Methods in molecular biology (Clifton, N.J.) Volume: 935 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
|
Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
|
Languages: ENG Pagination: 329-340 Citation Subset: - |
Affiliation:
|
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Double Chromatin Immunoprecipitation: Analysis of Target Co-occupancy of Retinal Transcription Facto...
Next Document: Optimized Technique for Subretinal Injections in Mice.