| Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas chromatography-mass spectrometry Application to polytraumatized patients. | |
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MedLine Citation:
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PMID: 18706873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F(2)-isoprostanes (F(2)-IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo. Since "interferences peaks" at m/z 573 co-elute with d(4)-15-F(2t)-IsoP preferentially used, we propose a new GC-NICI-MS approach to quantify urinary F(2)-IsoPs by using 4(RS)-F(4t)-neuroprostane as the internal standard. This method was applied to quantify urinary F(2)-IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase (p<0.0001) in urinary F(2)-IsoPs in polytraumatized patients compared with healthy volunteers (4.73+/-2.75 ng/mg vs. 0.811+/-0.359 ng/mg creatinine). |
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Authors:
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Emilie Mas; Françoise Michel; Alexandre Guy; Valérie Bultel; Yoan Falquet; Patrick Chardon; Jean-Claude Rossi; Jean Paul Cristol; Thierry Durand |
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Publication Detail:
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Type: Journal Article Date: 2008-08-03 |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 872 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-26 Completed Date: 2008-10-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 133-40 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Lapeyronie Hospital, Montpellier, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Case-Control Studies F2-Isoprostanes / urine* Gas Chromatography-Mass Spectrometry / methods* Humans Lipid Peroxidation Multiple Trauma / urine* Reference Standards Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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0/F2-Isoprostanes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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