Document Detail


Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry.
MedLine Citation:
PMID:  22990793     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, and the human malaria parasite, Plasmodium falciparum.
Authors:
Photini Sinnis; Patricia De La Vega; Alida Coppi; Urszula Krzych; Maria M Mota
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  923     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-19     Completed Date:  2013-01-30     Revised Date:  2014-03-17    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  385-400     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Anopheles / parasitology
Cell Culture Techniques
Cell Line
Cell Movement*
Flow Cytometry / methods*
Hep G2 Cells
Hepatocytes / parasitology*,  pathology
Humans
Malaria / parasitology
Mice
Microscopy / methods*
Plasmodium / growth & development*
Sporozoites / metabolism*
Grant Support
ID/Acronym/Agency:
R01 AI056840/AI/NIAID NIH HHS
Comments/Corrections

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