| Quantification of the sixth DNA base 5-hydroxymethylcytosine in colorectal cancer tissue and C-26 cell line. | |
MedLine Citation:
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PMID: 23534428 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: DNA methylation at the five position of cytosine is well recognized as an important epigenetic modification in human health and disease. Recent evidences demonstrated that 5-methylcytosine (5-mC) by the TET family of enzymes can be converted to 5-hydroxymethylcytosine (5-hmC). Here, we use an ultrasensitive and accurate isotope-based LC-MS/MS method to precisely determine the levels of 5-hmC and 5-mC in colorectal cancer and the C-26 colon adenocarcinoma cell line. Results: Our data showed that 5-hmC content is significantly reduced (approximately sixfold) in colorectal cancer as compared with adjacent normal tissue. Similarly, the ratio of 5-hmC to 5-mC dropped from 0.054 ± 0.005 in normal tissues, to 0.011 ± 0.002 in cancer. Conclusion: The analysis of 5-hmC levels and the ratio of 5-hmC:5-mC during tumor progression might provide insight into the role of this modification in cellular immortalization and transformation. |
Authors:
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Liang-Tao Zhang; Li-Jian Zhang; Jun-Jie Zhang; Xiao-Xia Ye; Ai-Mei Xie; Li-Yu Chen; Jing X Kang; Chun Cai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioanalysis Volume: 5 ISSN: 1757-6199 ISO Abbreviation: Bioanalysis Publication Date: 2013 Apr |
Date Detail:
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Created Date: 2013-03-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101512484 Medline TA: Bioanalysis Country: England |
Other Details:
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Languages: eng Pagination: 839-45 Citation Subset: IM |
Affiliation:
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Guangdong Medical College, Zhanjiang, Guangdong 524023, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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