Document Detail


Quantification of the sixth DNA base 5-hydroxymethylcytosine in colorectal cancer tissue and C-26 cell line.
MedLine Citation:
PMID:  23534428     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: DNA methylation at the five position of cytosine is well recognized as an important epigenetic modification in human health and disease. Recent evidences demonstrated that 5-methylcytosine (5-mC) by the TET family of enzymes can be converted to 5-hydroxymethylcytosine (5-hmC). Here, we use an ultrasensitive and accurate isotope-based LC-MS/MS method to precisely determine the levels of 5-hmC and 5-mC in colorectal cancer and the C-26 colon adenocarcinoma cell line. Results: Our data showed that 5-hmC content is significantly reduced (approximately sixfold) in colorectal cancer as compared with adjacent normal tissue. Similarly, the ratio of 5-hmC to 5-mC dropped from 0.054 ± 0.005 in normal tissues, to 0.011 ± 0.002 in cancer. Conclusion: The analysis of 5-hmC levels and the ratio of 5-hmC:5-mC during tumor progression might provide insight into the role of this modification in cellular immortalization and transformation.
Authors:
Liang-Tao Zhang; Li-Jian Zhang; Jun-Jie Zhang; Xiao-Xia Ye; Ai-Mei Xie; Li-Yu Chen; Jing X Kang; Chun Cai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioanalysis     Volume:  5     ISSN:  1757-6199     ISO Abbreviation:  Bioanalysis     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101512484     Medline TA:  Bioanalysis     Country:  England    
Other Details:
Languages:  eng     Pagination:  839-45     Citation Subset:  IM    
Affiliation:
Guangdong Medical College, Zhanjiang, Guangdong 524023, People's Republic of China.
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