| Quantification of selective phosphatidylserine oxidation during apoptosis. | |
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MedLine Citation:
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PMID: 15502241 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Membrane phospholipids are gaining increasing attention as important mediators in a variety of signal transduction processes. Oxidation and changes in membrane topography of lipids are probably important elements in the regulation of phospholipid-dependent signaling. Phosphatidylserine (PS), in particular, is implicated in the regulation of macrophage-dependent clearance of apoptotic cell "corpses" in a pathway probably mediated by selective oxidation and translocation of PS in the plasma membrane. Here we describe our highly sensitive and specific assay to measure differential lipid peroxidation in individual phospholipid classes in live cells using metabolic integration of the fluorescent oxidation-sensitive fatty acid analog cis-parinaric acid (cis-PnA) and resolution of specific phospholipids by high-performance liquid chromatography (HPLC). These experimental approaches can provide insight into the roles and mechanisms of PS oxidation in the identification and clearance of apoptotic cells. |
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Authors:
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James P Fabisiak; Yulia Y Tyurina; Vladimir A Tyurin; Valerian E Kagan |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 291 ISSN: 1064-3745 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2005 |
Date Detail:
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Created Date: 2004-10-25 Completed Date: 2005-02-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 449-56 Citation Subset: IM |
Affiliation:
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Department of Environmental and Occupational Health, University of Pittsburgh, PA, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Cell Membrane / chemistry Chromatography, High Pressure Liquid Fatty Acids, Unsaturated / analysis, metabolism Fluorescent Dyes Humans Jurkat Cells Lipid Peroxidation Oxidation-Reduction Oxidative Stress Phosphatidylserines / analysis*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 0/Fluorescent Dyes; 0/Phosphatidylserines; 18427-44-6/parinaric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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