Document Detail

Quantification and regulation of apolipoprotein E expression in rat Kupffer cells.
MedLine Citation:
PMID:  2723547     Owner:  NLM     Status:  MEDLINE    
Apolipoprotein E (apoE) is synthesized by a wide variety of cells including cells of the monocyte-macrophage lineage. In order to assess the quantitative significance of apoE synthesis in a mature tissue macrophage, apoE synthesis was compared in Kupffer cells and hepatocytes isolated from rat liver. Immunoreactive apoE synthesized by both cell types exhibited identical isoform patterns when examined by high-resolution two-dimensional gel analysis. ApoE synthesis was not detected in hepatic endothelial cells. Northern blot analysis using a rat apoE cDNA probe demonstrated a single mRNA species of approximately 1200 nucleotides in freshly isolated hepatocytes and Kupffer cells. The absolute content of apoE mRNA in each cell type was determined with a DNA-excess solution hybridization assay. The apoE mRNA content (pg/microgram RNA) for Kupffer cells and hepatocytes was 35.7 and 98.8, respectively. Accounting for cellular RNA content and the population size of each cell type in the liver, Kupffer cells were calculated to contain about 0.7% of liver apoE mRNA; hepatocytes account almost quantitatively for the remainder. These results suggest that Kupffer cells are not major contributors to the plasma apoE pool. After intravenous injection of bacterial endotoxin, apoE mRNA was decreased in freshly isolated Kupffer cells whereas whole liver showed no change in apoE mRNA. Endotoxin treatment had no effect on the apoE mRNA content in several peripheral tissues. These results indicate that apoE expression in vivo is differentially regulated by endotoxin in Kupffer cells as compared to hepatocytes or apoE-producing cells in peripheral tissues.
P A Dawson; L M Lukaszewski; P F Ells; C C Malbon; D L Williams
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  30     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-07-10     Completed Date:  1989-07-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  403-13     Citation Subset:  IM    
Department of Pharmacological Sciences, State University of New York, Stony Brook 11794-8651.
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MeSH Terms
Apolipoproteins B / metabolism
Apolipoproteins E / biosynthesis*
DNA Probes
Electrophoresis, Gel, Two-Dimensional
Endotoxins / pharmacology
Kupffer Cells / metabolism*
Liver / cytology
RNA, Messenger / analysis
Rats, Inbred Strains
Grant Support
Reg. No./Substance:
0/Apolipoproteins B; 0/Apolipoproteins E; 0/DNA Probes; 0/Endotoxins; 0/RNA, Messenger

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