| Quantification of plaque neovascularization using contrast ultrasound: a histologic validation. | |
| | |
MedLine Citation:
|
PMID: 20581005 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIMS: The density of vasa vasorum within atherosclerotic plaque correlates with histologic features of plaque vulnerability in post-mortem studies. Imaging methods to non-invasively detect vasa vasorum are limited. We hypothesized that contrast ultrasound (CUS) can quantify vasa vasorum during atherosclerosis progression. METHODS AND RESULTS: New Zealand white rabbits received a high-fat diet for 3 weeks, and bilateral femoral artery stenosis was induced by balloon injury. Contrast ultrasound femoral imaging was performed at baseline and 2, 4, and 6 weeks post injury to quantify adventitial videointensity. At each imaging time point 10 vessels were sectioned and stained with haematoxylin and eosin and von-Willebrand factor. Adventitial vasa vasorum density was quantified by counting the number of stained microvessels and their total cross-sectional area. Plaque size (per cent lumen area) progressed over time (P < 0.001), as did adventitial vasa vasorum density (P < 0.001). Plateau peak videointensity also progressed, demonstrating a strong linear correlation with histologic vasa vasorum density (P < 0.001). Receiver operating characteristic analysis indicated that a three-fold increase in median adventitial videointensity had a sensitivity of 100% and specificity of 88% for predicting abnormal neovascularization. CONCLUSION: We have histologically validated that CUS quantifies the development of adventitial vasa vasorum associated with atherosclerosis progression. This imaging technique has the potential for characterizing prognostically significant plaque features. |
| | |
Authors:
|
Diego Moguillansky; Xiaoping Leng; Andrew Carson; Linda Lavery; Abigail Schwartz; Xucai Chen; Flordeliza S Villanueva |
Related Documents
:
|
17822005 - Comparison of maximum intensity projection and digitally reconstructed radiographic pro... 12459425 - Detection of inflamed plaques with contrast ultrasound. 8127955 - Time-of-flight methods in mr angiography. 7603355 - A computerized method for determination of microvascular density. 20236885 - Sparse array 3-d isar imaging based on maximum likelihood estimation and clean technique. 20725255 - Snop: a method for skeletonization of a fringe pattern along the fringe direction. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Validation Studies Date: 2010-06-24 |
Journal Detail:
|
Title: European heart journal Volume: 32 ISSN: 1522-9645 ISO Abbreviation: Eur. Heart J. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-03-02 Completed Date: 2011-12-22 Revised Date: 2012-03-01 |
Medline Journal Info:
|
Nlm Unique ID: 8006263 Medline TA: Eur Heart J Country: England |
Other Details:
|
Languages: eng Pagination: 646-53 Citation Subset: IM |
Affiliation:
|
Center for Ultrasound Molecular Imaging and Therapeutics, University of Pittsburgh Cardiovascular Institute, Pittsburgh, PA, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Atherosclerosis / pathology*, ultrasonography Cell Proliferation Constriction, Pathologic / pathology, ultrasonography Diet, High-Fat Disease Progression Femoral Artery / pathology*, ultrasonography Neovascularization, Pathologic / pathology Plaque, Atherosclerotic / pathology*, ultrastructure Rabbits Ultrasonography, Interventional Vasa Vasorum / pathology*, ultrasonography |
| Grant Support | |
ID/Acronym/Agency:
|
R01HL077534/HL/NHLBI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Impact of competitive flow on wall shear stress in coronary surgery: computational fluid dynamics of...
Next Document: The Escherichia coli glycophage display system.