Document Detail

Quantification of phospholipids in infant formula and growing up milk by high-performance liquid chromatography with evaporative light scattering detector.
MedLine Citation:
PMID:  20629400     Owner:  NLM     Status:  MEDLINE    
Phospholipids (PLs) are well known for their excellent emulsifier properties and more recently for their biological functions, such as cell signing, brain development, immune function, heart health, and cancer prevention, besides their physiological role in membrane composition. In dairy products, PLs represent 0.2-1% of milk fat. The milk PLs comprise phosphatidylcholine (PC), phosphatidylethanolamine (PE), and sphingomyelin (SPH) as the major compounds; phosphatidylinositol and phosphatidylserine are minor PLs. A new generation of dairy products claiming PL family content, such as SPH, is being produced; therefore, a validated method for quantifying PL families in dairy products is needed. In this study, an HPLC-evaporative light scattering detector method to quantify the most abundant milk PL families, i.e., PC, PE, and SPH, in infant formula and growing up milk was developed and validated.
Marcel Braun; Brigitte Flück; Claudia Cotting; Florence Monard; Francesca Giuffrida
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 May-Jun
Date Detail:
Created Date:  2010-07-15     Completed Date:  2010-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  948-55     Citation Subset:  IM    
Nestlé Product Technology Centre, Konolfingen, Switzerland.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Infant Formula / chemistry*
Milk / chemistry*
Phospholipids / analysis*
Reproducibility of Results
Scattering, Radiation
Reg. No./Substance:

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