Document Detail

Quantification of pancreatic islet distribution in situ in mice.
MedLine Citation:
PMID:  19808908     Owner:  NLM     Status:  MEDLINE    
Tracing changes of specific cell populations in health and disease is an important goal of biomedical research. Precisely monitoring pancreatic beta-cell proliferation and islet growth is a challenging area of research. We have developed a method to capture the distribution of beta-cells in the intact pancreas of transgenic mice with fluorescence-tagged beta-cells with a macro written for ImageJ ( Total beta-cell area and islet number and size distribution are quantified with reference to specific parameters and location for each islet and for small clusters of beta-cells. The entire distribution of islets can now be plotted in three dimensions, and the information from the distribution on the size and shape of each islet allows a quantitative and a qualitative comparison of changes in overall beta-cell area at a glance.
German Kilimnik; Abraham Kim; Junghyo Jo; Kevin Miller; Manami Hara
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-10-06
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  297     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2009-12-23     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E1331-8     Citation Subset:  IM    
Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA.
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MeSH Terms
Green Fluorescent Proteins / biosynthesis,  chemistry,  genetics
Image Processing, Computer-Assisted / methods*
Immunohistochemistry / methods
Insulin / genetics
Insulin-Secreting Cells / cytology*,  metabolism
Mice, Transgenic
Promoter Regions, Genetic
Grant Support
Reg. No./Substance:
0/Ins1 protein, mouse; 0/Insulin; 147336-22-9/Green Fluorescent Proteins

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