Document Detail


Quantification of pancreatic islet distribution in situ in mice.
MedLine Citation:
PMID:  19808908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tracing changes of specific cell populations in health and disease is an important goal of biomedical research. Precisely monitoring pancreatic beta-cell proliferation and islet growth is a challenging area of research. We have developed a method to capture the distribution of beta-cells in the intact pancreas of transgenic mice with fluorescence-tagged beta-cells with a macro written for ImageJ (rsb.info.nih.gov/ij/). Total beta-cell area and islet number and size distribution are quantified with reference to specific parameters and location for each islet and for small clusters of beta-cells. The entire distribution of islets can now be plotted in three dimensions, and the information from the distribution on the size and shape of each islet allows a quantitative and a qualitative comparison of changes in overall beta-cell area at a glance.
Authors:
German Kilimnik; Abraham Kim; Junghyo Jo; Kevin Miller; Manami Hara
Related Documents :
11484918 - Transdifferentiation of human islet cells in a long-term culture.
19595468 - No mantle formation in rodent islets -- the prototype of islet revisited.
2227108 - Effect of bacitracin on retroendocytosis and degradation of insulin in cultured kidney ...
3292308 - Phospholipid metabolism of a-cell enriched pancreatic islets.
20047518 - The observation of phenotypic changes of schwann cells after rat sciatic nerve injury.
10984428 - Exosome: from internal vesicle of the multivesicular body to intercellular signaling de...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-10-06
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  297     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-25     Completed Date:  2009-12-23     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E1331-8     Citation Subset:  IM    
Affiliation:
Department of Medicine, The University of Chicago, Chicago, Illinois 60637, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Green Fluorescent Proteins / biosynthesis,  chemistry,  genetics
Image Processing, Computer-Assisted / methods*
Immunohistochemistry / methods
Insulin / genetics
Insulin-Secreting Cells / cytology*,  metabolism
Mice
Mice, Transgenic
Promoter Regions, Genetic
Grant Support
ID/Acronym/Agency:
DK-072473/DK/NIDDK NIH HHS; DK-081527/DK/NIDDK NIH HHS; DK-20595/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Ins1 protein, mouse; 0/Insulin; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Sodium glucose co-transporter 1 (SGLT1) mediates glucose-induced incretin secretion in mice in vivo.
Next Document:  Pigment epithelium-derived factor suppresses adipogenesis via inhibition of the MAPK/ERK pathway in ...