| Quantification of nonselective bulk autophagy in S. cerevisiae using Pgk1-GFP. | |
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MedLine Citation:
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PMID: 20523132 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rapid estimation of the macroautophagi crate has become of great importance over the past few years. A variety of methods to follow autophagy were established both in S. cerevisiae and the mammalian system. In yeast,measuring the breakdown of GFP-Atg8,and in mammalian cells counting the increase of LC3 puncta, have become the most commonly used assays to quantify autophagy. Here, we provide degradation of Pgk1-GFP followed in immunoblots as a new convenient tool to quantify nonselective bulk autophagy in yeast. |
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Authors:
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Evelyn Welter; Michael Thumm; Roswitha Krick |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Autophagy Volume: 6 ISSN: 1554-8635 ISO Abbreviation: Autophagy Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-25 Completed Date: 2010-12-01 Revised Date: 2011-06-30 |
Medline Journal Info:
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Nlm Unique ID: 101265188 Medline TA: Autophagy Country: United States |
Other Details:
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Languages: eng Pagination: 794-7 Citation Subset: IM |
Affiliation:
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Department Biochemistry II, University Medicine Goettingen, Georg-August University, Goettingen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autophagy* Biological Assay / methods* Blotting, Western Green Fluorescent Proteins / metabolism* Microscopy, Fluorescence Phosphoglycerate Kinase / metabolism* Protein Processing, Post-Translational Recombinant Fusion Proteins / metabolism* Saccharomyces cerevisiae / cytology*, metabolism* Vacuoles / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.7.2.3/Phosphoglycerate Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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