Document Detail

Quantification of nonselective bulk autophagy in S. cerevisiae using Pgk1-GFP.
MedLine Citation:
PMID:  20523132     Owner:  NLM     Status:  MEDLINE    
Rapid estimation of the macroautophagi crate has become of great importance over the past few years. A variety of methods to follow autophagy were established both in S. cerevisiae and the mammalian system. In yeast,measuring the breakdown of GFP-Atg8,and in mammalian cells counting the increase of LC3 puncta, have become the most commonly used assays to quantify autophagy. Here, we provide degradation of Pgk1-GFP followed in immunoblots as a new convenient tool to quantify nonselective bulk autophagy in yeast.
Evelyn Welter; Michael Thumm; Roswitha Krick
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Autophagy     Volume:  6     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-25     Completed Date:  2010-12-01     Revised Date:  2011-06-30    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  794-7     Citation Subset:  IM    
Department Biochemistry II, University Medicine Goettingen, Georg-August University, Goettingen, Germany.
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MeSH Terms
Biological Assay / methods*
Blotting, Western
Green Fluorescent Proteins / metabolism*
Microscopy, Fluorescence
Phosphoglycerate Kinase / metabolism*
Protein Processing, Post-Translational
Recombinant Fusion Proteins / metabolism*
Saccharomyces cerevisiae / cytology*,  metabolism*
Vacuoles / metabolism
Reg. No./Substance:
0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; EC Kinase

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