| Quantification of non-QB-reducing centers in leaves using a far-red pre-illumination. | |
MedLine Citation:
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PMID: 16049767 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An alternative approach to quantification of the contribution of non-QB-reducing centers to Chl a fluorescence induction curve is proposed. The experimental protocol consists of a far-red pre-illumination followed by a strong red pulse to determine the fluorescence rise kinetics. The far-red pre-illumination induces an increase in the initial fluorescence level (F(25 micros)) that saturates at low light intensities indicating that no light intensity-dependent accumulation of QA - occurs. Far-red light-dose response curves for the F(25 micros)-increase give no indication of superimposed period-4 oscillations. F(25 micros)-dark-adaptation kinetics following a far-red pre-pulse, reveal two components: a faster one with a half-time of a few seconds and a slower component with a half-time of around 100 s. The faster phase is due to the non-QB-reducing centers that re-open by recombination between QA - and the S-states on the donor side. The slower phase is due to the recombination between QB - and the donor side in active PS II reaction centers. The pre-illumination-induced increase of the F(25 micros)-level represents about 4-5% of the variable fluorescence for pea leaves ( approximately 2.5% equilibrium effect and 1.8-3.0% non-QB-reducing centers). For the other plant species tested these values were very similar. The implications of these values will be discussed. |
Authors:
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Gert Schansker; Reto J Strasser |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Photosynthesis research Volume: 84 ISSN: 0166-8595 ISO Abbreviation: Photosyn. Res. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-07-28 Completed Date: 2006-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100954728 Medline TA: Photosynth Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 145-51 Citation Subset: IM |
Affiliation:
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Bioenergetics Laboratory, University of Geneva, Chemin des Embrouchis 10, 1254, Jussy, Geneva, Switzerland. Gert.Schansker@bioen.unige.ch |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chlorophyll
/
chemistry,
metabolism Color Dose-Response Relationship, Radiation Ficus / metabolism, radiation effects Fluorescence Light* Peas / chemistry, metabolism, radiation effects Photochemistry Photosystem II Protein Complex / chemistry, metabolism Plant Leaves / metabolism*, radiation effects* Species Specificity |
| Chemical | |
Reg. No./Substance:
|
0/Photosystem II Protein Complex; 1406-65-1/Chlorophyll; 479-61-8/chlorophyll a |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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