Document Detail


Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses.
MedLine Citation:
PMID:  10960827     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Achiral and chiral semi-micro column high-performance liquid chromatographic methods with fluorescence detection to determine methamphetamine and amphetamine in human hair are described. These compounds were extracted into 5% trifluoroacetic acid (TFA) in methanol, derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride and separated either on a 250 x 1.5 mm i.d. octadecyl-silane (ODS) or a 150 x 2 mm i.d. OD-RH column. Linear calibration curves extending over a wide range of concentration that covers the practical samples were obtained for amphetamine, methamphetamine and their enantiomers (r = 0.999). Resolution values for amphetamine and methamphetamine enantiomers were 3.4 and 1.1, respectively. Intra- and inter-day variations of both the methods were not larger than 8.9% expressed as relative standard deviations (n >/= 5). The limits of detection at a signal-to-noise ratio of 3 obtained by both the methods were in the range of 1.0-4.7 fmol/5 microL injection with the achiral method being more sensitive. Abusers' hair samples were analyzed by the two methods and only the S(+)-enantiomers were found in eight Japanese abusers' hair samples. The achiral method was used to study the concentrations of these compounds in single black and white hair strands of abusers.
Authors:
O Y Al-Dirbashi; N Kuroda; M Wada; M Takahashi; K Nakashima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  14     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-25     Completed Date:  2000-09-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  293-300     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Affiliation:
Department of Analytical Research for Pharmacoinformatics, Graduate School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amphetamine / analysis*,  chemistry
Calibration
Chromatography, High Pressure Liquid / instrumentation,  methods*
Hair / chemistry*
Humans
Methamphetamine / analysis*,  chemistry
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence
Stereoisomerism
Substance Abuse Detection / methods*
Chemical
Reg. No./Substance:
300-62-9/Amphetamine; 537-46-2/Methamphetamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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