Document Detail

Quantification of methamphetamine, amphetamine and enantiomers by semi-micro column HPLC with fluorescence detection; applications on abusers' single hair analyses.
MedLine Citation:
PMID:  10960827     Owner:  NLM     Status:  MEDLINE    
Achiral and chiral semi-micro column high-performance liquid chromatographic methods with fluorescence detection to determine methamphetamine and amphetamine in human hair are described. These compounds were extracted into 5% trifluoroacetic acid (TFA) in methanol, derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)-benzoyl chloride and separated either on a 250 x 1.5 mm i.d. octadecyl-silane (ODS) or a 150 x 2 mm i.d. OD-RH column. Linear calibration curves extending over a wide range of concentration that covers the practical samples were obtained for amphetamine, methamphetamine and their enantiomers (r = 0.999). Resolution values for amphetamine and methamphetamine enantiomers were 3.4 and 1.1, respectively. Intra- and inter-day variations of both the methods were not larger than 8.9% expressed as relative standard deviations (n >/= 5). The limits of detection at a signal-to-noise ratio of 3 obtained by both the methods were in the range of 1.0-4.7 fmol/5 microL injection with the achiral method being more sensitive. Abusers' hair samples were analyzed by the two methods and only the S(+)-enantiomers were found in eight Japanese abusers' hair samples. The achiral method was used to study the concentrations of these compounds in single black and white hair strands of abusers.
O Y Al-Dirbashi; N Kuroda; M Wada; M Takahashi; K Nakashima
Related Documents :
561117 - Analysis of formaldehyde in tobacco smoke by high performance liquid chromatography.
12215677 - Identification of pyridine compounds in cigarette smoke solution that inhibit growth of...
9330037 - Derivatization of amphetamine and methamphetamine with 1,2-naphthoquinone 4-sulfonic ac...
12380807 - Determination of nicotine and other minor alkaloids in international cigarettes by soli...
3022217 - Biodistribution in rats of 99tcm-labelled human serum albumin.
929197 - Polychlorobornane components of toxaphene: structure-toxicity relations and metabolic r...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  14     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-25     Completed Date:  2000-09-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  293-300     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Ltd.
Department of Analytical Research for Pharmacoinformatics, Graduate School of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amphetamine / analysis*,  chemistry
Chromatography, High Pressure Liquid / instrumentation,  methods*
Hair / chemistry*
Methamphetamine / analysis*,  chemistry
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence
Substance Abuse Detection / methods*
Reg. No./Substance:
300-62-9/Amphetamine; 537-46-2/Methamphetamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Determination of amobarbital and secobarbital in plasma samples using micellar liquid chromatography...
Next Document:  Capillary electrophoretic analysis of brimonidine in aqueous humor of the eye and blood sera and rel...