| Quantification and mechanisms of oleic acid-induced steatosis in HepG2 cells. | |
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MedLine Citation:
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PMID: 20182586 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Developing a quantifiable in vitro model of steatosis is critical in understanding the pathogenesis of nonalcoholic fatty liver disease (NAFLD) and searchingfor effective therapies. Using an ORO-based colorimetric measurement, we developed a convenient assay to qualify the degree of OA-induced steatosis in HepG2 cells. We demonstrated that in the absence of exogenous inflammatory mediators, OA-induced steatosis was associated with increased production and secretion of tumor necrosis factor alpha and decreased expression of peroxisome proliferators-activated receptor alpha in HepG2 cells. OA-induced steatosis was also associated with increased lipid peroxidation, apoptosis, but decreased proliferation in these cells. The increased lipid peroxidation was related to decreased SOD-1, a free radical scavenger enzyme; while increased apoptosis was related to increased active caspase-9. The decreased proliferation mediated by OA-induced steatosis was associated with increased production of p27 with unchanged alanine transaminase (ALT) level in the culture medium, indicating OA-induced steatosis alters cell cycle progression without direct toxicity to these cells. In conclusion, the present study developed a colorimetric assay that accurately quantifies OA-induced steatosis in HepG2 cells. In the absence of exogenous inflammatory mediators, OA-induced steatosis results in a series of pathophysilogical changes in HepG2 cells, indicating direct pathogenic roles of hepatocytes in NAFLD. |
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Authors:
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Wei Cui; Stephen L Chen; Ke-Qin Hu |
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Publication Detail:
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Type: Journal Article Date: 2010-01-01 |
Journal Detail:
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Title: American journal of translational research Volume: 2 ISSN: 1943-8141 ISO Abbreviation: Am J Transl Res Publication Date: 2010 |
Date Detail:
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Created Date: 2010-02-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101493030 Medline TA: Am J Transl Res Country: United States |
Other Details:
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Languages: eng Pagination: 95-104 Citation Subset: - |
Affiliation:
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Division of Gastroenterology and Hepatology, Dept. of Medicine, University of California Irvine, CA, USA. |
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