| Quantification of lipoic acid in plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry. | |
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MedLine Citation:
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PMID: 16112626 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sensitive and specific liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) method has been developed and validated for the identification and quantification of lipoic acid (LA) in human plasma. LA and the internal standard, naproxen, were extracted from a 500 microl plasma sample by one-step deproteination using acetonitrile. Chromatographic separation was performed on a Zorbax SB-C(18) Column (100 mmx3.0mm i.d. with 3.5 microm particle size) with the mobile phase consisting of acetonitrile and 0.1% acetic acid (pH 4, adjusted with ammonia solution) (65:35, v/v), and the flow rate was set at 0.3 ml/min. Detection was performed on a single quadrupole mass spectrometer by selected ion monitoring (SIM) mode via electrospray ionization (ESI) source. The method was linear over the concentration range of 5-10,000 ng/ml for LA. The intra- and inter-day precisions were less than 7% and accuracy ranged from -7.87 to 9.74% at the LA concentrations tested. The present method provides a relatively simple and sensitive assay with short turn-around time. The method has been successfully applied to a clinical pharmacokinetic study of LA in 10 healthy subjects. |
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Authors:
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Jun Chen; Wenming Jiang; Jia Cai; Weixing Tao; Xiaoling Gao; Xinguo Jiang |
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Publication Detail:
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Type: Clinical Trial; Journal Article |
Journal Detail:
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Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 824 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-08-30 Completed Date: 2005-10-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 249-57 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutics, School of Pharmacy, Fudan University, 138 Yi Xue Yuan Road, BOX 130, Fenglin Campus, Shanghai 200032, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Antioxidants / administration & dosage, pharmacokinetics Area Under Curve Calibration Chromatography, High Pressure Liquid / methods* Half-Life Humans Reproducibility of Results Spectrometry, Mass, Electrospray Ionization / methods* Thioctic Acid / administration & dosage, blood*, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 62-46-4/Thioctic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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