Document Detail


Quantification of lipids and protein in thin biofilms by fluorescence staining.
MedLine Citation:
PMID:  18409111     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The efficiency of removing unwanted biofilm from surfaces in industrial water systems was examined by fluorescence microscopy and image analysis. A quantitative assay for in situ determination of biofilm components was developed and tested on thin biofilms grown in reactors as well as real biofilms sampled from a fish processing factory. Different fluorescent dyes for in situ detection of protein, lipid and total organic matter were tested. It was possible to determine the approximate amounts, concentrations and coverage of the different components by correlating the fluorescent intensity of the biofilm components to standard solutions immobilised as a biofilm. The quantification methods were evaluated as a strategy for determining the efficiency of different disinfection/cleaning procedures, showing that quantification of these biofilm components was fast and reliable for optimisation of cleaning in place procedures. However, the approach also showed that bacterial cells, as investigated by culture-independent procedures, were killed but not removed by most disinfection procedures tested, potentially leading to surfaces which are easily recolonised.
Authors:
Poul Larsen; Bo Højris Olesen; Per Halkjaer Nielsen; Jeppe Lund Nielsen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biofouling     Volume:  24     ISSN:  1029-2454     ISO Abbreviation:  Biofouling     Publication Date:  2008  
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-09-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9200331     Medline TA:  Biofouling     Country:  England    
Other Details:
Languages:  eng     Pagination:  241-50     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Aalborg, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / metabolism*
Bacterial Proteins / chemistry*
Biofilms / growth & development*
Fishes
Fluorescent Dyes*
Food Industry
Image Processing, Computer-Assisted
Lipids / chemistry*
Microscopy, Fluorescence
Organic Chemicals
Oxazines
Staining and Labeling
Stainless Steel
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Fluorescent Dyes; 0/Lipids; 0/NanoOrange; 0/Organic Chemicals; 0/Oxazines; 12597-68-1/Stainless Steel; 7385-67-3/nile red

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