Document Detail


Quantification of isotope-labelled and unlabelled folates in plasma, ileostomy and food samples.
MedLine Citation:
PMID:  21052651     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
New stable isotope dilution assays were developed for the simultaneous quantitation of [(13)C(5)]-labelled and unlabelled 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, folic acid along with unlabelled tetrahydrofolic acid and 10-formylfolic acid in clinical samples deriving from human bioavailability studies, i.e. plasma, ileostomy samples, and food. The methods were based on clean-up by strong anion exchange followed by LC-MS/MS detection. Deuterated analogues of the folates were applied as the internal standards in the stable isotope dilution assays. Assay sensitivity was sufficient to detect all relevant folates in the respective samples as their limits of detection were below 0.62 nmol/L in plasma and below 0.73 μg/100 g in food or ileostomy samples. Quantification of the [(13)C(5)]-label in clinical samples offers the possibility to differentiate between folate from endogenous body pools and the administered dose when executing bioavailability trials.
Authors:
Barbara E Büttner; Veronica E Öhrvik; Cornelia M Witthöft; Michael Rychlik
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-30
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  399     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  429-39     Citation Subset:  IM    
Affiliation:
Division BIOANALYTIK Weihenstephan, Research Center for Nutrition and Food Sciences (Z I E L), Technische Universität München, 85350 Freising-Weihenstephan, Germany.
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