| Quantification of individual oligosaccharide compounds from human milk using high-pH anion-exchange chromatography. | |
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MedLine Citation:
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PMID: 8833329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A quantification method suitable for determination of individual oligosaccharide compounds from human milk has been established. The crude milk oligosaccharide fraction was separated into acidic oligosaccharides, neutral oligosaccharides, and lactose by gel permeation chromatography. After this separation step neutral and acidic oligosaccharides were analyzed separately by high-pH anion-exchange chromatography with pulsed amperometric detection. The concentrations of 14 neutral oligosaccharides, of 6 acidic oligosaccharides, and of N-acetylneuraminic acid were determined on the internal standards stachyose and galacturonic acid, respectively. Thus, previously applied quantification methods for milk oligosaccharides based on gel permeation chromatography have been decisively improved. |
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Authors:
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S Thurl; B Muller-Werner; G Sawatzki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 235 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1996 Mar |
Date Detail:
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Created Date: 1997-05-30 Completed Date: 1997-05-30 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 202-6 Citation Subset: IM |
Affiliation:
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Research Department, Milupa GmbH & Co., Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gel Chromatography, Ion Exchange / methods* Electrochemistry Humans Hydrogen-Ion Concentration Milk, Human / chemistry* Oligosaccharides / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Oligosaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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