Document Detail

Quantification of individual oligosaccharide compounds from human milk using high-pH anion-exchange chromatography.
MedLine Citation:
PMID:  8833329     Owner:  NLM     Status:  MEDLINE    
A quantification method suitable for determination of individual oligosaccharide compounds from human milk has been established. The crude milk oligosaccharide fraction was separated into acidic oligosaccharides, neutral oligosaccharides, and lactose by gel permeation chromatography. After this separation step neutral and acidic oligosaccharides were analyzed separately by high-pH anion-exchange chromatography with pulsed amperometric detection. The concentrations of 14 neutral oligosaccharides, of 6 acidic oligosaccharides, and of N-acetylneuraminic acid were determined on the internal standards stachyose and galacturonic acid, respectively. Thus, previously applied quantification methods for milk oligosaccharides based on gel permeation chromatography have been decisively improved.
S Thurl; B Muller-Werner; G Sawatzki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  235     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1997-05-30     Completed Date:  1997-05-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  202-6     Citation Subset:  IM    
Research Department, Milupa GmbH & Co., Germany.
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MeSH Terms
Chromatography, Gel
Chromatography, Ion Exchange / methods*
Hydrogen-Ion Concentration
Milk, Human / chemistry*
Oligosaccharides / analysis*
Reg. No./Substance:

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