Document Detail


Quantification of high-density-lipoprotein cholesterol by precipitation with phosphotungstic acid/MgCl2.
MedLine Citation:
PMID:  6640896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We evaluated the use of a modified phosphotungstic acid/MgCl2 precipitation procedure for the precipitation of apolipoprotein B-containing lipoproteins. Precipitation of these lipoproteins [very-low- and low-density lipoproteins, and lipoprotein (a)] is complete, with negligible coprecipitation of high-density lipoprotein subfractions (HDL1, HDL2, HDL3), even in hypertriglyceridemic sera. In comparison with ultracentrifugation, the precipitation method yields, on the average, values that are 0.17 mmol/L lower for cholesterol values but almost identical for apolipoprotein A-I and phosphatidylcholine. Looking for delta 3,5-cholestadiene formed from cholesterol in the precipitation residue, we used "high-performance" liquid chromatography and "high-performance" thin-layer chromatography and found none.
Authors:
G Assmann; H Schriewer; G Schmitz; E O Hägele
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2026-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Apolipoproteins / isolation & purification
Apolipoproteins B
Chemical Precipitation
Cholestadienes / isolation & purification
Cholesterol / blood*,  isolation & purification
Cholesterol, HDL
Evaluation Studies as Topic
Humans
Lipoproteins, HDL / blood*,  isolation & purification
Magnesium*
Magnesium Chloride
Phosphotungstic Acid*
Statistics as Topic
Triglycerides / blood
Ultracentrifugation
Chemical
Reg. No./Substance:
0/Apolipoproteins; 0/Apolipoproteins B; 0/Cholestadienes; 0/Cholesterol, HDL; 0/Lipoproteins, HDL; 0/Triglycerides; 12067-99-1/Phosphotungstic Acid; 57-88-5/Cholesterol; 7439-95-4/Magnesium; 7786-30-3/Magnesium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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