| Quantification of heterocyclic amine carcinogens in cooked meats using isotope dilution liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry. | |
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MedLine Citation:
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PMID: 9364795 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heterocyclic aromatic amines (HAAs) are formed in cooked meats through pyrolysis reactions of different amino acids in the presence or absence of creatine/creatinine and sugars. HAAs are mutagens, colon/mammary gland carcinogens in rodents, and are suspected in the etiology of human cancers. In this study, cooked meats containing incurred HAAs as well as control (microwave) meat, were spiked with four labeled HAA internal standards (MeIQx, IQ, AAC and PhIP) and extracted using a liquid/liquid cleanup procedure. Isotope dilution measurements were made using on-line liquid chromatography atmosphere pressure chemical ionization tandem mass spectrometry with multiple reaction monitoring to provide the sensitivity and specificity needed for trace analysis in these complex matrices. The procedure was validated using control meat spiked with the four native HAAs at 0-50 ppb. The levels of HAAs found in cooked meats ranged from non-detectable (limit of detection 0.1-1.0 ppb) in microwave-cooked hamburger to 226 ppb PhIP and 104 ppb AAC in well-done grilled chicken. This methodology has the potential to provide accurate data on the consumption of HAAs in the diet for use in human cancer risk assessment. |
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Authors:
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C L Holder; S W Preece; S C Conway; Y M Pu; D R Doerge |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Rapid communications in mass spectrometry : RCM Volume: 11 ISSN: 0951-4198 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 1997 |
Date Detail:
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Created Date: 1997-12-22 Completed Date: 1997-12-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1667-72 Citation Subset: IM |
Affiliation:
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National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amines
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analysis* Animals Carcinogens / analysis* Cattle Chickens Chromatography, Liquid Cookery Heterocyclic Compounds / analysis* Humans Indicators and Reagents Mass Spectrometry Meat / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Amines; 0/Carcinogens; 0/Heterocyclic Compounds; 0/Indicators and Reagents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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