| Quantification of ferritin from staple food crops. | |
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MedLine Citation:
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PMID: 19292462 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ferritin-iron has been shown to be as bioavailable as ferrous sulfate in humans. Thus, biofortification to breed crops with high ferritin content is a promising strategy to alleviate the global iron deficiency problem. Although ferritin is present in all food crops, its concentration varies between species and varieties. Therefore, a successful ferritin biofortification strategy requires a method to rapidly measure ferritin concentrations in food crops. The objective of this study was to develop a simple and reliable ELISA using an anti-ferritin polyclonal antibody to detect ferritin in various crops. Crude seed extracts were found to have 10.2 +/- 1.0, 4.38 +/- 0.9, 1.2 +/- 0.3, 0.38 +/- 0.1, and 0.04 +/- 0.01 microg of ferritin/g of dry seed in red beans, white beans, wheat, maize, and brown rice, respectively. Although the measured absolute concentrations of ferritin values were low, the presented method is applicable for rapid screening for the relative ferritin concentrations of large numbers of seeds to identify and breed ferritin-rich crops. |
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Authors:
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Rebecca J Lukac; Maneesha R Aluru; Manju B Reddy |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 57 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-18 Completed Date: 2009-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 2155-61 Citation Subset: IM |
Affiliation:
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Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Breeding Crops, Agricultural / chemistry* Enzyme-Linked Immunosorbent Assay / methods Ferritins / analysis*, chemistry Food Analysis Immunoblotting Molecular Sequence Data Oryza sativa / chemistry Phaseolus / chemistry Seeds / chemistry Sequence Alignment Triticum / chemistry Zea mays / chemistry |
| Chemical | |
Reg. No./Substance:
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9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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