Document Detail


Quantification of ferritin from staple food crops.
MedLine Citation:
PMID:  19292462     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ferritin-iron has been shown to be as bioavailable as ferrous sulfate in humans. Thus, biofortification to breed crops with high ferritin content is a promising strategy to alleviate the global iron deficiency problem. Although ferritin is present in all food crops, its concentration varies between species and varieties. Therefore, a successful ferritin biofortification strategy requires a method to rapidly measure ferritin concentrations in food crops. The objective of this study was to develop a simple and reliable ELISA using an anti-ferritin polyclonal antibody to detect ferritin in various crops. Crude seed extracts were found to have 10.2 +/- 1.0, 4.38 +/- 0.9, 1.2 +/- 0.3, 0.38 +/- 0.1, and 0.04 +/- 0.01 microg of ferritin/g of dry seed in red beans, white beans, wheat, maize, and brown rice, respectively. Although the measured absolute concentrations of ferritin values were low, the presented method is applicable for rapid screening for the relative ferritin concentrations of large numbers of seeds to identify and breed ferritin-rich crops.
Authors:
Rebecca J Lukac; Maneesha R Aluru; Manju B Reddy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  57     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-18     Completed Date:  2009-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2155-61     Citation Subset:  IM    
Affiliation:
Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Breeding
Crops, Agricultural / chemistry*
Enzyme-Linked Immunosorbent Assay / methods
Ferritins / analysis*,  chemistry
Food Analysis
Immunoblotting
Molecular Sequence Data
Oryza sativa / chemistry
Phaseolus / chemistry
Seeds / chemistry
Sequence Alignment
Triticum / chemistry
Zea mays / chemistry
Chemical
Reg. No./Substance:
9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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