| Quantification of the emetic toxin cereulide in food products by liquid chromatography-mass spectrometry using synthetic cereulide as a standard. | |
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MedLine Citation:
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PMID: 20889798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacillus cereus produces the emetic toxin cereulide, a cyclic dodecadepsipeptide that can act as a K(+) ionophore, dissipating the transmembrane potential in mitochondria of eukaryotic cells. Because pure cereulide has not been commercially available, cereulide content in food samples has been expressed in valinomycin equivalents, a highly similar cyclic potassium ionophore that is commercially available. This research tested the biological activity of synthetic cereulide and validated its use as a standard in the quantification of cereulide contents in food samples. The synthesis route consists of 10 steps that result in a high yield of synthetic cereulide that showed biological activity in the HEp-2 cell assay and the boar sperm motility assay. The activity is different in both methods, which may be attributed to differences in K(+) content of the test media used. Using cereulide or valinomycin as a standard to quantify cereulide based on liquid chromatography-mass spectrometry (LC-MS), the concentration determined with cereulide as a standard was on average 89.9% of the concentration determined using valinomycin as a standard. The recovery experiments using cereulide-spiked food products and acetonitrile as extraction solute showed that the LC-MS method with cereulide as a standard is a reliable and accurate method to quantify cereulide in food, because the recovery rate was close to 100% over a wide concentration range. |
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Authors:
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Elisabeth G Biesta-Peters; Martine W Reij; Richard H Blaauw; Paul H In 't Veld; Andreja Rajkovic; Monika Ehling-Schulz; Tjakko Abee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-01 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 76 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-08 Completed Date: 2011-02-22 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 7466-72 Citation Subset: IM |
Affiliation:
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Wageningen University and Research Centre, Laboratory of Food Microbiology, EV Wageningen, Netherlands. els.biesta-peters@wur.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Survival / drug effects Chromatography, Liquid / methods*, standards Depsipeptides / analysis*, chemical synthesis, toxicity Emetics / analysis*, chemical synthesis, toxicity Food Analysis / methods* Hepatocytes / drug effects Humans Locomotion / drug effects Male Mass Spectrometry / methods*, standards Reference Standards Spermatozoa / drug effects Sus scrofa |
| Chemical | |
Reg. No./Substance:
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0/Depsipeptides; 0/Emetics; 0/cereulide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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