| Quantification of elastase-like activity in 13 human cancer cell lines and in an immortalized human epithelial cell line by RP-HPLC. | |
| | |
MedLine Citation:
|
PMID: 12817479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A sensitive and specific RP-HPLC assay was developed to measure the levels of polymorphonuclear elastase (PMN-E) activity in growing cell cultures. By combining a pre-incubation of the cells with a relatively non-toxic, PMN-E-specific inhibitor, MeOSuc-Ala-Ala-Pro-Val-chloromethylketone (MAAPVCK), the p-nitroaniline formed by the hydrolysis of the substrate MeOSuc-Ala-Ala-Pro-Val-p-NA by PMN-E is quantified. Elastase-like activity was measured in 14 human cells lines: 13 cancer cell lines (HL-60, U-937, A-427, LCLC-103H, YAPC, DAN-G, PA-TU-8902, KYSE-70, -510, -520, 5637, SISO and MCF-7) and one immortalized epithelial cell line (hTert-RPE1). Activity was detected in all lines; the lowest was found in hTert-RPE1 cells while the highest was detected in a pancreas adenocarcinoma line (PA-TU-8902). When the results were normalized according to cell volume instead of cell number, the leukemia line HL-60 had the highest activity and PA-TU-8902 ranked second. A 1 h pre-incubation with 9.0 microM of the irreversible PMN-E inhibitor MAAPVCK led to varying degrees of enzyme inhibition depending on the cell line; the strongest inhibition was observed with the PA-TU-8902 pancreatic cancer cell line (90% inhibition) while the weakest was seen with the A-427 lung cancer cell line (52%). These results indicate that PA-TU-8902 is a suitable in vitro model for testing the efficacy of PMN-E-activated prodrugs of antitumor agents. |
| | |
Authors:
|
Karin Achilles; Patrick J Bednarski |
Related Documents
:
|
17623109 - Complement susceptibility in glutamine deprived breast cancer cells. 7558719 - Thrombin increases expression of urokinase receptor by activation of the thrombin recep... 20931209 - The plasminogen activator inhibitor system in colon cancer cell lines is influenced by ... 3258609 - Binding of staphylococcal enterotoxin a to accessory cells is a requirement for its abi... 1600129 - Immune damage to the mesangium: antibody- and complement-mediated stimulation and destr... 3944459 - Binding of c-reactive protein to nucleated cells leads to complement activation without... 8442019 - Alterations in cytoskeletal protein sulfhydryls and cellular glutathione in cultured ce... 10717239 - Enhancement of the transformed shape phenotype by microtubule inhibitors and reversal b... 3345729 - Correlation of spatial differences in concentrations of prolactin and growth hormone ce... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biological chemistry Volume: 384 ISSN: 1431-6730 ISO Abbreviation: Biol. Chem. Publication Date: 2003 May |
Date Detail:
|
Created Date: 2003-06-23 Completed Date: 2004-02-10 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 817-24 Citation Subset: IM |
Affiliation:
|
Institute of Pharmacy, Ernst-Moritz-Arndt University, D-17487 Greifswald, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aniline Compounds
/
analysis Cell Division / drug effects, physiology Cell Line Cell Line, Tumor Cell Size Chromatography, High Pressure Liquid / methods Enzyme Inhibitors / pharmacology Epithelial Cells / cytology, metabolism Humans Leukocyte Elastase / antagonists & inhibitors, metabolism* Neoplasms / enzymology* |
| Chemical | |
Reg. No./Substance:
|
0/Aniline Compounds; 0/Enzyme Inhibitors; 100-01-6/4-nitroaniline; EC 3.4.21.37/Leukocyte Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-in...
Next Document: Differences in the activation mechanism between the alpha and beta subunits of human meprin.