| Quantification of cortical GABA-glutamine cycling rate using in vivo magnetic resonance signal of [2-13C]GABA derived from glia-specific substrate [2-13C]acetate. | |
| | |
MedLine Citation:
|
PMID: 17056156 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Brain [2-(13)C]gamma-aminobutyric acid (GABA) signal derived from the glia-specific substrate [2-(13)C]acetate reflects the extent of the GABA-glutamine neurotransmitter cycling between GABAergic neurons and glial cells. We report, for the first time, in vivo quantification of the GABA-glutamine cycling flux. The GABA-glutamine cycling flux rate was determined to be 1.8+/-0.4 micromol/(gh) (mean+/-S.D., n=6, approximately 6% of total tricarboxylic acid cycle rate) in the neocortex of vigabatrin-treated rats. The relatively small magnitude of glial contribution to the clearance of extracellular GABA measured in this study provided in vivo evidence to support the concept of a significant neuronal reuptake of GABA, which short-circuits the GABA-glutamine cycling pathway for repletion of released neurotransmitter GABA. |
| | |
Authors:
|
Jehoon Yang; Shizhe S Li; John Bacher; Jun Shen |
Related Documents
:
|
14579796 - Different actions of gamma-hydroxybutyrate: a critical outlook. 3794716 - Deuterium isotope effect in gamma-aminobutyric acid transamination: determination of ra... 8389066 - Effect of a toxin isolated from the sponge haliclona viridis on the release of gamma-am... 17209546 - Characterization of the aminocarboxycyclopropane-forming enzyme cmac. 9363366 - Inhibition of k+ transport in human sickle cell erythrocytes by okadaic acid and sodium... 21733326 - Resveratrol attenuates steatosis in obese zucker rats by decreasing fatty acid availabi... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Intramural Date: 2006-10-23 |
Journal Detail:
|
Title: Neurochemistry international Volume: 50 ISSN: 0197-0186 ISO Abbreviation: Neurochem. Int. Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2007-01-22 Completed Date: 2007-03-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8006959 Medline TA: Neurochem Int Country: England |
Other Details:
|
Languages: eng Pagination: 371-8 Citation Subset: IM |
Affiliation:
|
National Institute of Mental Health Intramural Research Program, 9000 Rockville Pike, Bethesda, MD, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetates
/
metabolism Animals Cerebral Cortex / cytology, metabolism* Cyclohexanecarboxylic Acids / pharmacology Enzyme Inhibitors / pharmacology Female Glucose / metabolism Glutamine / metabolism* Kinetics Macaca mulatta Magnetic Resonance Spectroscopy Male Neuroglia / metabolism* Rats Rats, Sprague-Dawley Vigabatrin / pharmacology gamma-Aminobutyric Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Acetates; 0/Cyclohexanecarboxylic Acids; 0/Enzyme Inhibitors; 50-99-7/Glucose; 56-12-2/gamma-Aminobutyric Acid; 56-85-9/Glutamine; 60643-86-9/Vigabatrin; 87980-11-8/gabaculine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differential regulation of calcium channel coding genes by prolonged depolarization.
Next Document: LPA-mediated demyelination in ex vivo culture of dorsal root.