| Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia. | |
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MedLine Citation:
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PMID: 18201283 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PROBLEM: To better understand innate immune responses to sexually-transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll-like receptor (TLR) expression from a population of women is needed. METHOD OF STUDY: TLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration. RESULTS: TLR1-3 and 5-9 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7-9 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels. CONCLUSION: TLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling. |
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Authors:
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Melissa M Herbst-Kralovetz; Alison J Quayle; Mercedes Ficarra; Sheila Greene; William A Rose; Ralph Chesson; Rae Ann Spagnuolo; Richard B Pyles |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-01-12 |
Journal Detail:
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Title: American journal of reproductive immunology (New York, N.Y. : 1989) Volume: 59 ISSN: 1046-7408 ISO Abbreviation: Am. J. Reprod. Immunol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-15 Completed Date: 2009-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8912860 Medline TA: Am J Reprod Immunol Country: Denmark |
Other Details:
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Languages: eng Pagination: 212-24 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555-0436, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Cervix Uteri / cytology, immunology, metabolism* Chemokine CCL2 / immunology, metabolism Cyclopropanes Diglycerides / immunology Epithelium / immunology, metabolism Female Flagellin Gene Expression Profiling Guanosine / analogs & derivatives Humans Immunity, Innate* Immunity, Mucosal Interleukin-1beta / immunology, metabolism Interleukin-6 / immunology, metabolism Interleukin-8 / immunology, metabolism Lipopolysaccharides Oligopeptides / immunology RNA, Double-Stranded Sexually Transmitted Diseases / immunology Toll-Like Receptors / biosynthesis*, immunology Vagina / cytology, immunology, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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T-32 AI0762605/AI/NIAID NIH HHS; U19 AI61972/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CCL2; 0/Cyclopropanes; 0/Diglycerides; 0/FSL-1 lipoprotein, synthetic; 0/Interleukin-1beta; 0/Interleukin-6; 0/Interleukin-8; 0/Lipopolysaccharides; 0/N2-cyclopropylamine-guanosine; 0/Oligopeptides; 0/RNA, Double-Stranded; 0/Toll-Like Receptors; 118-00-3/Guanosine; 12777-81-0/Flagellin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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