Document Detail


Quantification of chemotactic response of quiescent and proliferating fibroblasts in Boyden chambers by computer-assisted image analysis.
MedLine Citation:
PMID:  1987269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chemotactic response of human gingival fibroblasts to platelet-derived growth factor (PDGF) was investigated in 48-well modified Boyden chambers. Results were quantified using computer-assisted image analysis of propidium iodidestained cells and were compared with results obtained using the conventional method of quantification by direct counting. Quantification by image analysis was found to be rapid and accurate, and correlated closely with results obtained by direct counting. Bromodeoxyuridine (BrdU)-labeled proliferating cells were double stained with propidium iodide and fluorescein isothiocyanate (FITC)-conjugated anti-BrdU antiserum. The cycling cells showed a markedly reduced chemotactic response to PDGF, whereas cells in S-phase of the cell cycle did not show any response. This method of quantification of results of Boyden chamber assays is simple and reliable, and allows the use of double labels to investigate the chemotactic response of subpopulations of cells within a heterogeneous population of cells.
Authors:
F J Hughes; C A McCulloch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  39     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-02-21     Completed Date:  1991-02-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  243-6     Citation Subset:  IM    
Affiliation:
MRC Group in Dental Implantology, Faculty of Dentistry, University of Toronto, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle / drug effects
Cells, Cultured
Chemotaxis / drug effects*
Fibroblasts / drug effects,  immunology
Gingiva / cytology,  drug effects,  immunology*
Humans
Image Processing, Computer-Assisted
Platelet-Derived Growth Factor / pharmacology*
Chemical
Reg. No./Substance:
0/Platelet-Derived Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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