Document Detail


Quantification of the cellular proliferation on freshly dispersed cells from rat anterior pituitaries after in vivo and in vitro labelling with bromodeoxyuridine.
MedLine Citation:
PMID:  1374748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The labelling index i.e., the proportion of cells in S phase of the cell cycle, has been calculated in cytospin preparations of rat anterior pituitary cells after labelling either in vivo or in vitro with the thymidine analogue bromodeoxyuridine (BrdU). The aims of this work were (1) to check whether enzymatic digestion interferes with the incorporation of BrdU into S phase cells and/or whether it has any deletereous effect on the immunohistochemical detection of cells that have already incorporated BrdU, and (2) to check the viability of simultaneous staining for BrdU and markers for the different types of pituitary cells in the cytospins. No statistical difference was found between the labelling index after in vivo or in vitro labelling with BrdU. Identification of doubly-immunostained cells was straightforward and up to 40% of BrdU-labelled cells were immunopositive for pituitary hormones. It is suggested that cytospin preparations from biopsy samples may be used to study cellular proliferation without exposing the patient to the hazardous effects of BrdU infusion and without the interference of cell culture methods.
Authors:
S Carbajo; F Gonzalez del Pozo; E Carbajo-Perez
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Histochemical journal     Volume:  24     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-06-17     Completed Date:  1992-06-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  137-43     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Histology, Faculty of Medicine, University of Salamanca, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromodeoxyuridine*
Cell Division*
Female
Male
Pituitary Gland, Anterior / cytology
Rats
Staining and Labeling*
Trypsin
Chemical
Reg. No./Substance:
59-14-3/Bromodeoxyuridine; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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