Document Detail


Quantification of breast cancer cells in peripheral blood samples by real-time rt-PCR.
MedLine Citation:
PMID:  23225441     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers.
MATERIALS AND METHODS: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. RNA was isolated from the samples and transcribed into cDNA. TaqMan real-time PCR for cytokeratins 8, 18 and 19 was carried out and was correlated to that of 18S.
RESULTS: Cytokeratin gene expression increased in all samples, when as few as 10 tumour cells were added. In the CAMA-1 cell line, the increase was even greater the more cells were added.
CONCLUSION: By this methodology, cells from mammary carcinoma cell lines can be detected in blood samples. Its benefit will be validated in samples from patients with breast cancer.
Authors:
Michael Zebisch; Alexandra C Kölbl; Christian Schindlbeck; Julia Neugebauer; Sabine Heublein; Matthias Ilmer; Brigitte Rack; Klaus Friese; Udo Jeschke; Ulrich Andergassen
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  32     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  5387-91     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, Ludwig-Maximilians-University of Munich, Maistrasse 11, D-80337 Munich, Germany. udo.jeschke@med.uni-muenchen.de.
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