Document Detail


Quantification of bile acids directly from urine by MALDI-TOF-MS.
MedLine Citation:
PMID:  12638043     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability to quantify mixtures of bile acids using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry directly from urine has been demonstrated. Six cholic acid derivatives were selected for analysis: taurocholic acid (TCA), taurochenodeoxycholic acid (TCDCA), taurolithocholic acid (TLCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), and glycolithocholic acid (GCDCA). Urine samples were pre-concentrated and purified using solid-phase extraction (SPE) columns. The method was optimized to eliminate suppression effects, and proved to be reproducible from day to day. Calibration curves averaged from three days were obtained for the bile acids directly from urine, and then tested for their ability to accurately determine concentrations from one measurement. In summary, a simple, rapid method has been developed for the quantification of bile salts from urine with SPE clean-up by MALDI-MS.
Authors:
DebraLynn Mims; David Hercules
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2003-02-22
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  375     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-14     Completed Date:  2003-04-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  609-16     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Vanderbilt University, Nashville, TN 37214, USA.
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MeSH Terms
Descriptor/Qualifier:
Bile Acids and Salts / urine*
Calibration
Humans
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Chemical
Reg. No./Substance:
0/Bile Acids and Salts

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