Document Detail

Quantification and application of the placental epigenetic signature of the RASSF1A gene in maternal plasma.
MedLine Citation:
PMID:  20661892     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The aims of the detection of hypermethylated RASSF1A gene in maternal plasma from all three trimesters of pregnancy were to show its feature of cell-free fetal DNA and to make up deficients of genetic markers. This study also aimed at investigating of its application value in pre-eclampsia compared with third trimester.
METHODS: Eighty pregnant women (7-41 gestational weeks) including normal pregnant women (60 cases) and pre-eclamptic pregnant women (20 cases) were selected as study groups and 20 normal non-pregnant women were selected as control group. Free DNA of plasma samples was extracted, and RASSF1A levels before and after double-methylation-sensitive restriction enzyme digestion of HinP1I and HhaI were determined to measure total and fetal cell-free DNA, respectively. beta-Actin gene was detected as a control to confirm complete enzyme digestion.
RESULTS: The median concentrations of hypermethylated RASSF1A gene were 46 copies/mL in first trimester, 96 copies/mL in second trimester, 527 copies/mL in third trimester and 1947 copies/mL in pre-eclampsia. There was positive correlation between fetal-derived hypermethylated RASSF1A levels and the severity of pre-eclampsia.
CONCLUSION: Hypermethylated RASSF1A gene may be considered as an epigenetic marker to detect the fetal DNA in maternal plasma and expands the clinical application of non-invasive prenatal diagnosis.
Fuxi Zhao; Jian Wang; Runhua Liu; Jing Yang; Ke Cui; Yongming Wu; Juncheng Guo; Yaqin Mu; Xiying Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  30     ISSN:  1097-0223     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-27     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  778-82     Citation Subset:  IM    
Copyright Information:
(c) 2010 John Wiley & Sons, Ltd.
Institute of Immunology, Medical College of Shanxi Datong University, Datong, China.
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MeSH Terms
DNA / genetics,  metabolism
DNA Methylation / genetics
Deoxyribonucleases, Type II Site-Specific / metabolism
Epigenesis, Genetic / genetics*
Gestational Age
Polymerase Chain Reaction
Pre-Eclampsia / blood,  genetics*
Prenatal Diagnosis / methods*
Statistics as Topic
Tumor Suppressor Proteins / blood,  genetics*
Reg. No./Substance:
0/RASSF1 protein, human; 0/Tumor Suppressor Proteins; 9007-49-2/DNA; EC, Type II Site-Specific; EC type II deoxyribonucleases

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