| Quantification and application of the placental epigenetic signature of the RASSF1A gene in maternal plasma. | |
| | |
MedLine Citation:
|
PMID: 20661892 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVES: The aims of the detection of hypermethylated RASSF1A gene in maternal plasma from all three trimesters of pregnancy were to show its feature of cell-free fetal DNA and to make up deficients of genetic markers. This study also aimed at investigating of its application value in pre-eclampsia compared with third trimester. METHODS: Eighty pregnant women (7-41 gestational weeks) including normal pregnant women (60 cases) and pre-eclamptic pregnant women (20 cases) were selected as study groups and 20 normal non-pregnant women were selected as control group. Free DNA of plasma samples was extracted, and RASSF1A levels before and after double-methylation-sensitive restriction enzyme digestion of HinP1I and HhaI were determined to measure total and fetal cell-free DNA, respectively. beta-Actin gene was detected as a control to confirm complete enzyme digestion. RESULTS: The median concentrations of hypermethylated RASSF1A gene were 46 copies/mL in first trimester, 96 copies/mL in second trimester, 527 copies/mL in third trimester and 1947 copies/mL in pre-eclampsia. There was positive correlation between fetal-derived hypermethylated RASSF1A levels and the severity of pre-eclampsia. CONCLUSION: Hypermethylated RASSF1A gene may be considered as an epigenetic marker to detect the fetal DNA in maternal plasma and expands the clinical application of non-invasive prenatal diagnosis. |
| | |
Authors:
|
Fuxi Zhao; Jian Wang; Runhua Liu; Jing Yang; Ke Cui; Yongming Wu; Juncheng Guo; Yaqin Mu; Xiying Wang |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Prenatal diagnosis Volume: 30 ISSN: 1097-0223 ISO Abbreviation: Prenat. Diagn. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-07-27 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8106540 Medline TA: Prenat Diagn Country: England |
Other Details:
|
Languages: eng Pagination: 778-82 Citation Subset: IM |
Copyright Information:
|
(c) 2010 John Wiley & Sons, Ltd. |
Affiliation:
|
Institute of Immunology, Medical College of Shanxi Datong University, Datong, China. dtyzfx@163.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
DNA
/
genetics,
metabolism DNA Methylation / genetics Deoxyribonucleases, Type II Site-Specific / metabolism Epigenesis, Genetic / genetics* Female Gestational Age Humans Polymerase Chain Reaction Pre-Eclampsia / blood, genetics* Pregnancy Prenatal Diagnosis / methods* Statistics as Topic Tumor Suppressor Proteins / blood, genetics* |
| Chemical | |
Reg. No./Substance:
|
0/RASSF1 protein, human; 0/Tumor Suppressor Proteins; 9007-49-2/DNA; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific; EC 3.1.21.4/GCGC-specific type II deoxyribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Women's experience of pre-implantation genetic diagnosis: a qualitative study.
Next Document: Changes in phenolic compounds and Rancimat stability of olive oils from varieties of olives at diffe...