| Quantification of the amount of galacturonic acid residues in blocksequences in pectin homogalacturonan by enzymatic fingerprinting with exo- and endo-polygalacturonase II from Aspergillus niger. | |
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MedLine Citation:
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PMID: 10945680 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method to determine the amount of galacturonic acid in blocksequence (BS) in pectin homogalacturonan (HG) is described. The method is based on a combination of endopolygalacturonase II (endo-PG II) and exopolygalacturonase (exo-PG) digestion followed by quantification of the liberated galacturonic acid monomer. The amount of monomers released is directly related to the amount of non-esterified galacturonic acid units located between two other non-esterified galacturonic acids units on the HG chain. The amount released for exo-PG digestion only corresponds to the BS located at the non-reducing end of the polymer. The difference between total- and exo-BS was calculated to be the amount of endo-BS located either within or on the reducing end of the HG. Three series of model pectins obtained by de-esterification of a high-ester pectin with either plant pectin methyl-esterase (p-PME, P-series), fungal pectin methyl-esterase (f-PME, F-series) and chemical de-esterification using base (B-series) were analysed and compared with a fully de-esterified pectic acid sample obtained from the same raw material. Clear differences for the increase of the amounts of blocksequence could be seen between de-esterification of the P- and F-series samples supporting a blockwise and a homogenous de-esterification mechanism, respectively. f-PME and base treatment showed only minor differences in the increase of galacturonic acid units in BS, despite differences seen in their methyl-esterification pattern. Differences between the amounts of galacturonic acid located in exo- and endo-BS, provided evidence for the need of a certain start side or blocklength for p-PME to de-esterify blockwise. |
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Authors:
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G Limberg; R Körner; H C Buchholt; T M Christensen; P Roepstorff; J D Mikkelsen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 327 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-12-15 Completed Date: 2000-12-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 321-32 Citation Subset: IM |
Affiliation:
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Danisco Biotechnology, Copenhagen K, Denmark. gl@avh.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aspergillus niger
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enzymology* Carbohydrate Conformation Carbohydrate Sequence Hexuronic Acids / analysis* Models, Molecular Molecular Sequence Data Pectins / chemistry*, metabolism* Polygalacturonase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Hexuronic Acids; 0/Pectins; 14982-50-4/galacturonic acid; 9046-38-2/polygalacturonic acid; EC 3.2.1.-/endopolygalacturonase II; EC 3.2.1.15/Polygalacturonase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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