| Quantification of amino acids in a single cell by microchip electrophoresis with chemiluminescence detection. | |
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MedLine Citation:
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PMID: 22125158 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Analyzing individual cells allows detecting a minor group of abnormal cells present in a large population of normal cells. This ability can be essential to understanding diseases, such as cancer and diabetes. Microchip electrophoresis (MCE) is the technique of choice for single-cell analysis. However, since the channels in microfluidic devices are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe an MCE method with highly sensitive chemiluminescence detection for simultaneous determination of multiple amino acids present in single cells. |
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Authors:
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Yi-Ming Liu; Shulin Zhao |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 828 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 351-8 Citation Subset: IM |
Affiliation:
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Department of Chemistry and Biochemistry, Jackson State University, Jackson, MS, USA, yiming.liu@jsums.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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