Document Detail


Quantification of underivatized fatty acids from vegetable oils by HPLC with UV detection.
MedLine Citation:
PMID:  20819296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We propose a chromatographic method for the separation of saturated and unsaturated fatty acids by a high-performance liquid chromatography system, equipped with a photo diode array detector. Central to the method is the use of an appropriate mobile phase composed of acetonitrile, methanol, and n-hexane in ratio 90:8:2 acidified with 0.2% acetic acid, which allows the detection of fatty acids without a preliminary derivatization with chromophores or fluorescent dyes. Calibration on solutions of standards mixtures gives a quantification limit (at a wavelength of 208 nm) of 0.232, 0.093, 0.039, 0.056, 0.068, 0.004, 0.0005, 0.067 mg/mL for the myristic, palmitic, palmitoleic, stearic, oleic, linoleic, linolenic, and erucic acids, respectively. The method, applied to different vegetable oils (olive, sunflower, soybean, and palm) was able to distinguish the main fatty acids and quantify their amount. Data reliability was tested by comparing our results (on the relative percentages of some fatty acids in the olive oil) with those obtained by gas chromatographic analysis. Differences of the order of 0.3%, 0.6%, 2%, and 6% were observed for the oleic, linoleic, palmitic, and linolenic acids. Although less accurate, our method proved to be a simple alternative to standard gas chromatographic technique, as it can be applied even using a simple UV detector.
Authors:
V Guarrasi; M R Mangione; V Sanfratello; V Martorana; D Bulone
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  48     ISSN:  1945-239X     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-07     Completed Date:  2010-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  663-8     Citation Subset:  IM    
Affiliation:
Istituto di Biofisica (Palermo Unit), Consiglio Nazionale delle Ricerche (CNR) Via U. La Malfa 153 - 90146 Palermo, Italy.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid / methods*
Fatty Acids* / analysis,  isolation & purification
Fatty Acids, Unsaturated / analysis,  isolation & purification
Food Analysis / methods*
Linear Models
Plant Oils / chemistry*
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Plant Oils

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