Document Detail


Quantification of Salmonella in Food Samples from India Using the MINI-MSRV MPN and Modified MINI-MSRV MPN Methods.
MedLine Citation:
PMID:  22417597     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Salmonella is one of the most important food-borne pathogens. The MINI-MSRV MPN method was modified by replacing the isolation and confirmation of Salmonella on selective chromogenic agar with PCR/RT-PCR. This modification reduced the time for quantification by the MINI-MSRV MPN method by 24 h. Ninety-seven different food samples, comprising chicken, mutton, fish, and sprouts from different markets in Mumbai, India, were analyzed for quantification of Salmonella species by the MINI-MSRV MPN and modified MINI-MSRV MPN methods. Seventy-four percent of the chicken samples were found positive for Salmonella. However, Salmonella was found to be absent in fish, mutton, and sprouts samples. Salmonella load in the chicken sample was in the range of 1.30 to 120 MPN/g. This genotypic confirmation has advantage over variable phenotypic confirmation of pathogens.
Authors:
Ravindranth Shashidhar; Ishaa Srivastava; Jayant R Bandekar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food science     Volume:  76     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2012-03-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M564-7     Citation Subset:  IM    
Copyright Information:
© 2011 Institute of Food Technologists®
Affiliation:
Authors Shashidhar and Bandekar are with Food Technology Div., Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. Author Srivastava is with Dept. of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil Univ., 400 614 Navi Mumbai, India. Direct inquiries to author Bandekar (E-mail: jrb@barc.gov.in).
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