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Quantification of Individual Flagellate - Bacteria Interactions within Semi-natural Biofilms.
MedLine Citation:
PMID:  22186014     Owner:  NLM     Status:  Publisher    
Here we present a new approach to quantify food-web interactions within semi-natural biofilms by combining the establishment of biofilms from natural rivers in flow cells with video microscopy. In a first application of this approach, we focused on the surface-gliding heterotrophic flagellates (HF) Neobodo designis, Rhynchomonas nasuta and Planomonas sp. It was shown that the three HF generally ingested single biofilm-associated bacteria whereas bacteria within microcolonies were attacked but not ingested. However, grazing strategies differed considerably. While the kinetoplastids N. designis and R. nasuta displayed long search and short handling times, Planomonas sp. showed the opposite grazing characteristics. The latter behaviour resulted in a high relative predation success of 80% (precent of attacked prey ingested), whereas the relative predation success of the two kinetoplastids was only 20%. However, the two contrasting strategies resulted in similar ingestion rates for Planomonas sp. and N. designis of 0.5 to 0.6 ingestions flagellates(-1) minute(-1), respectively. Our results showed distinct differences in the feeding behaviour of three flagellates having similar life forms and provide direct evidence that microcolony formation in biofilms protects bacteria from grazing by HF in situ. The new approach provides individual-based insights into the complex food web interactions within biofilms.
Martina Erken; Nicole Farrenschon; Sophia Speckmann; Hartmut Arndt; Markus Weitere
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-18
Journal Detail:
Title:  Protist     Volume:  -     ISSN:  1618-0941     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806488     Medline TA:  Protist     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier GmbH. All rights reserved.
Helmholtz Centre for Environmental Research - UFZ, Department River Ecology, Brückstraße 3a, 39114 Magdeburg, Germany; University of Cologne, Cologne Biocenter, Zoological Institute, Department General Ecology, Zülpicher Straße 47b, D-50674 Köln, Germany.
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