Document Detail


Quantification of 3-methylglutaconic acid in urine, plasma, and amniotic fluid by isotope-dilution gas chromatography/mass spectrometry.
MedLine Citation:
PMID:  8111960     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described for quantification of the trace metabolite, 3-methylglutaconic acid, by isotope-dilution gas chromatography/mass spectrometry using synthetic 3-[2,4,6-13C3]methylglutaconic acid. Results are shown for quantification of 3-methylglutaconic acid in plasma, urine, cerebrospinal fluid and amniotic fluid for both normal controls and patients with different forms of 3-methylglutaconic aciduria. A simple method for the synthesis and purification of 3-[2,4,6-13C3]methylglutaconic acid is also described.
Authors:
R I Kelley
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  220     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1994-03-28     Completed Date:  1994-03-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
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MeSH Terms
Descriptor/Qualifier:
Adult
Amniotic Fluid / chemistry
Child
Child, Preschool
Gas Chromatography-Mass Spectrometry / methods*
Glutarates / analysis*,  blood,  cerebrospinal fluid,  urine
Humans
Infant
Grant Support
ID/Acronym/Agency:
DK-44933/DK/NIDDK NIH HHS; HD-07107/HD/NICHD NIH HHS; HD-10981/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Glutarates; 5746-90-7/3-methylglutaconic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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