| Quality not quantity for transglutaminase antibody 2: the performance of an endomysial and tissue transglutaminase test in screening coeliac disease remains stable over time. | |
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MedLine Citation:
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PMID: 23199329 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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National Institute of Clinical Excellence (NICE) and European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) guidance for the diagnosis of coeliac disease has been published. However, there is some controversy regarding the advice on the use of stratifying levels of immunoglobulin (IgA) tissue transglutaminase antibody (TG2) test positivity in the absence of test standardization and the vagueness of the indication to test equivocal samples. Using repeat service audit, we demonstrate that a combination of TG2 followed by IgA endomysial antibodies (EMA) is the best strategy for all degrees of mucosal abnormality using our test combination. Reliance upon immunoassay titre is not as effective, and cannot be applied consistently across populations in the absence of assay standardization. Guidelines advocating the use of tests should involve experts in laboratory diagnostics and external quality assurance to ensure that errors of generalization do not occur and that test performance is achievable in routine diagnostic use. |
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Authors:
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K Swallow; G Wild; R Sargur; D S Sanders; I Aziz; A D Hopper; W Egner |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 171 ISSN: 1365-2249 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2012-12-03 Completed Date: 2013-02-19 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 100-6 Citation Subset: IM |
Copyright Information:
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© 2012 British Society for Immunology. |
Affiliation:
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Immunology Department and Protein Reference Unit, Northern General Hospital, Sheffield, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autoantibodies
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analysis*,
immunology Celiac Disease / diagnosis*, immunology Clinical Audit Diagnostic Errors Enzyme-Linked Immunosorbent Assay GTP-Binding Proteins / immunology* Humans Immunoglobulin A / analysis, immunology Immunoglobulin G / analysis, immunology Intestinal Mucosa / chemistry, immunology Mass Screening / methods* Muscle Fibers, Skeletal / chemistry, immunology Quality Control Sensitivity and Specificity Serologic Tests Transglutaminases / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Immunoglobulin A; 0/Immunoglobulin G; EC 2.3.2.-/transglutaminase 2; EC 2.3.2.13/Transglutaminases; EC 3.6.1.-/GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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