| Quality evaluation of frozen apheresis red blood cell storage with 21-day postthaw storage in additive solution 3 and saline-adenine-glucose-mannitol: biochemical and chromium-51 recovery measures. | |
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MedLine Citation:
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PMID: 20051061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The Czech Republic decided to build the frozen red blood cell (RBC) bank for military contingency and civil emergency preparedness. Storage methods were validated with (51)Cr recovery measures out to 21 days postthaw. STUDY DESIGN AND METHODS: A total of 139 apheresis RBC units collected in additive solution (additive solution 3 [AS-3] or saline-adenine-glucose-mannitol [SAG-M]) were obtained from volunteers by double erythrocytapheresis; all were sterilely glycerolized, frozen, stored for at least 30 days at a minimum of -65 degrees C in collection or cryogenic bags, thawed, sterilely deglycerolized, and stored at 4 +/- 2 degrees C for up to 21 days in AS-3 or SAG-M. Units were sampled before storage and after thaw weekly. Metabolic intermediates and (51)Cr recovery measures were obtained to determine the index of therapeutic effectiveness (ITE). RESULTS: Despite losses associated with cryoconservation and eventual transfer to cryogenic bags, 3-week storage with optimum ITE was obtained with frozen storage in primary collection or cryogenic bags and postthaw storage in AS-3. Such cells would pass as acceptable in vitro hematology and biochemistry variables. CONCLUSIONS: A system for frozen RBC storage with 3-week, postthaw, liquid storage has been validated. With commercially available components and automated processing, it is capable of supporting civilian rare blood and emergency and international military combat and peacekeeping support use patterns. |
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Authors:
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Milos Bohonek; Marek Petr?s; Ivo Turek; Jaroslava Urbanov?; Tom?s Hr?dek; Petr Chm?tal; Vera Staroprazsk?; Jitka Kost?rov?; Dana Horcickov?; Simona Duchkov?; Jitka Svobodov?; Eva Tejckov? |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-28 |
Journal Detail:
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Title: Transfusion Volume: 50 ISSN: 1537-2995 ISO Abbreviation: Transfusion Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-06-15 Completed Date: 2010-07-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417360 Medline TA: Transfusion Country: United States |
Other Details:
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Languages: eng Pagination: 1007-13 Citation Subset: IM |
Affiliation:
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Department of Haematology, Biochemistry and Blood Transfusion, Central Military Hospital Prague, Prague, Czech Republic. milos.bohonek@uvn.cz |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenine* Adolescent Adult Blood Component Removal* Blood Preservation / standards* Chromium Radioisotopes / diagnostic use* Citrates* Erythrocytes / metabolism* Female Freezing Glucose* Hemoglobins / analysis Humans Male Middle Aged Phosphates* Sodium Chloride* |
| Chemical | |
Reg. No./Substance:
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0/AS-3 preservative solution; 0/Chromium Radioisotopes; 0/Citrates; 0/Hemoglobins; 0/Phosphates; 0/SAG-M blood; 50-99-7/Glucose; 73-24-5/Adenine; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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